产氨短杆菌的脂肪酸合成酶：多酶复合体催化单不饱和脂肪酸的形成。
Fatty acid synthetase from Brevibacterium ammoniagenes: formation of monounsaturated fatty acids by a multienzyme complex.
作者信息
Kawaguchi A, Okuda S
出版信息
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3180-3. doi: 10.1073/pnas.74.8.3180.
Abstract
A multienzyme fatty acid synthetase complex isolated from Brevibacterium ammoniagenes has been purified to a specific activity of 1440 nmol of malonyl-CoA incorporated per min/mg. The enzyme is homogeneous, as judged by gel electrophoresis on agarose gels, and has a molecular weight of 1.2 X 10(6). Both NADPH and NADH are required for activity. In contrast to other fatty acid synthetase complexes, the enzyme catalyzes the synthesis of both long-chain saturated and monounsaturated fatty acids from malonyl-CoA and acetyl-CoA. The formation of unsaturated fatty acids is oxygen-independent and sharply reduced by 3-decynoyl-N-acetylcysteamine, a known inhibitor of Escherchia coli beta-hydroxydecanoyl thioester dehydrase (EC 4.2.1.60).
摘要
从产氨短杆菌中分离出的一种多酶脂肪酸合成酶复合物已被纯化至比活性为每分钟每毫克掺入1440 nmol丙二酰辅酶A。通过琼脂糖凝胶电泳判断，该酶是均一的，分子量为1.2×10⁶。活性需要NADPH和NADH两者。与其他脂肪酸合成酶复合物不同，该酶催化从丙二酰辅酶A和乙酰辅酶A合成长链饱和脂肪酸和单不饱和脂肪酸。不饱和脂肪酸的形成不依赖氧气，并被3-癸炔酰-N-乙酰半胱氨酸显著降低，3-癸炔酰-N-乙酰半胱氨酸是大肠杆菌β-羟基癸酰硫酯脱水酶（EC 4.2.1.60）的已知抑制剂。
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Proc Natl Acad Sci U S A. 1977 Aug;74(8):3180-3. doi: 10.1073/pnas.74.8.3180.
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