嗜热栖热菌HB8来源的TaqI同裂酶。
An isochizomer of TaqI from Thermus thermophilus HB8.
作者信息
Sato S, Shinomiya T
出版信息
J Biochem. 1978 Nov;84(5):1319-21. doi: 10.1093/oxfordjournals.jbchem.a132252.
Abstract
A site-specific endonuclease has been isolated from Thermus thermophilus HB8 and named TthHB8I. It recognizes the same sequences as TaqI from Thermus aquaticus YT-1 does. The amount of TthHB8I in the cells was comparable to that of TaqI. T. thermophilus HB8 has an advantage over T. aquaticus YT-1 for preparation of a TaqI-like enzyme since it is easier to obtain T. thermophilus HB8 cells in quantity.
摘要
一种位点特异性核酸内切酶已从嗜热栖热菌HB8中分离出来，并命名为TthHB8I。它识别的序列与嗜热水栖菌YT-1中的TaqI相同。细胞中TthHB8I的量与TaqI相当。由于更容易大量获得嗜热栖热菌HB8细胞，因此在制备类似TaqI的酶方面，嗜热栖热菌HB8比嗜热水栖菌YT-1具有优势。
Zotero 插件