Allred M C, Dunmire D L
J Chromatogr Sci. 1978 Nov 10;16(11):533-7. doi: 10.1093/chromsci/16.11.533.
A method for the determination of sulfamethazine at the 0.5 ppm level by high performance liquid chromatography (HPLC) has been shortened and simplified by use of a mini-column clean-up procedure. Fortified feed samples were extracted with acetonitrile, concentrated, and redissolved in the mobile phase. Sulfamethazine was separated from most extraneous material by a mini-column of C18/Porasil B and AX/Corasil, and analyzed by HPLC at 254 nm. Liquid standard solutions were used to fortify four formulated swine feeds and corn sample each at three levels, giving an average recovery of 98% and a relative standard deviation of 7.3%. Tests were run using 32 antibiotics and drugs to determine if they would interfere. Most of these substances were not eluted under the conditions used. Compounds which absorbed at the wavelength used did not interfere with the sulfamethazine peak.
一种通过高效液相色谱法（HPLC）测定0.5 ppm水平磺胺二甲嘧啶的方法，因采用微型柱净化程序而得到缩短和简化。强化饲料样品用乙腈萃取、浓缩，然后重新溶解于流动相中。磺胺二甲嘧啶通过C18/Porasil B微型柱和AX/Corasil微型柱与大多数外来物质分离，并在254 nm处通过HPLC进行分析。使用液体标准溶液对四种配方猪饲料和玉米样品分别进行三个水平的强化，平均回收率为98%，相对标准偏差为7.3%。使用32种抗生素和药物进行测试以确定它们是否会产生干扰。这些物质中的大多数在所用条件下未被洗脱。在所用波长处有吸收的化合物不会干扰磺胺二甲嘧啶峰。
