玉米和乳制品饲料中黄曲霉毒素的高压液相色谱测定及荧光检测
High pressure liquid chromatographic determination and fluorescence detection of aflatoxins in corn and dairy feeds.
作者信息
Cohen H, Lapointe M
出版信息
J Assoc Off Anal Chem. 1981 Nov;64(6):1372-6.
A high pressure liquid chromatographic (HPLC) method is described for the determination of aflatoxins B1, B2, G1, and G2 in animal feeds at levels as low as 2.5 ppb. Samples are extracted with acetonitrile-water and initially purified by using a Sep-Pak silica cartridge. The aflatoxins are then reacted with trifluoracetic acid and acetonitrile-water (1 + 1). After filtration, the aflatoxins are completely resolved on a 10 micrometer C18 column using a radial compression separation system with an acetonitrile-water solvent system. Aflatoxins G2a, B2a, G2, and B2 were reported in less than 30 min, using fluorescence detection. The method was successfully applied to samples at levels of 20-2.5 ppb added aflatoxins with recoveries in the range of 82-99%.
描述了一种高压液相色谱（HPLC）方法，用于测定动物饲料中低至2.5 ppb水平的黄曲霉毒素B1、B2、G1和G2。样品用乙腈 - 水萃取，最初使用Sep - Pak硅胶柱进行纯化。然后黄曲霉毒素与三氟乙酸和乙腈 - 水（1 + 1）反应。过滤后，使用带有乙腈 - 水溶剂系统的径向压缩分离系统，在10微米C18柱上完全分离黄曲霉毒素。使用荧光检测，在不到30分钟内报告了黄曲霉毒素G2a、B2a、G2和B2。该方法成功应用于添加了20 - 2.5 ppb黄曲霉毒素的样品，回收率在82 - 99%范围内。
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