Wilson D M
Arch Environ Contam Toxicol. 1989 May-Jun;18(3):308-14. doi: 10.1007/BF01062353.
Aflatoxin determinations can be approached many ways. Peanuts and corn are more often contaminated with aflatoxins B1 and B2 than with aflatoxins B1, B2, G1, and G2. Some countries are only interested in B1 content and others are interested in the total aflatoxin content. It is essential to safely handle all experimental materials associated with aflatoxin analyses or the aflatoxigenic fungi. Visual screening of suspect peanut lots, based on the presence of conidial heads of the Aspergillus flavus group, and screening corn for the presence of bright greenish yellow fluorescence (BGYF) are not chemical tests and such screening techniques may allow aflatoxin contaminated lots into commerce. Minicolumn screening procedures should always be used in conjunction with a quantitative method. Several thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) methods are suitable for quantitation and are in general use. Immunochemical methods such as the ELISA or affinity column methods are being rapidly developed. The chemical and immunochemical methods can be reliable if care is taken, using suitable controls and personnel that are well-trained. All analytical laboratories should stress safety and include suitable analytical validation procedures.
黄曲霉毒素的测定可以通过多种方式进行。花生和玉米受黄曲霉毒素B1和B2污染的情况比受黄曲霉毒素B1、B2、G1和G2污染的情况更为常见。一些国家只关注B1的含量，而其他国家则关注黄曲霉毒素的总含量。安全处理与黄曲霉毒素分析或产黄曲霉毒素真菌相关的所有实验材料至关重要。基于黄曲霉群分生孢子头的存在对可疑花生批次进行目视筛选，以及筛选玉米中是否存在亮黄绿色荧光（BGYF）并非化学检测，且此类筛选技术可能会使受黄曲霉毒素污染的批次进入商业流通。微柱筛选程序应始终与定量方法结合使用。几种薄层色谱（TLC）和高效液相色谱（HPLC）方法适用于定量分析，且普遍被使用。免疫化学方法如酶联免疫吸附测定（ELISA）或亲和柱方法正在迅速发展。如果小心操作，使用合适的对照并配备训练有素的人员，化学和免疫化学方法可以是可靠的。所有分析实验室都应强调安全性并纳入合适的分析验证程序。
