Honma K, Manabe H, Tomita M, Hamada A
J Biochem. 1981 Oct;90(4):1187-96. doi: 10.1093/oxfordjournals.jbchem.a133571.
A macroglycolipid containing galactose and N-acetylglucosamine as predominant sugar constituents was prepared together with glycophorin from rabbit erythrocyte membranes by extraction with lithium diiodosalicylate and partition in aqueous phenol. The macroglycolipid was effectively separated from the glycophorin fraction by ion-exchange chromatography in the presence of a detergent, Ammonyx LO. Its yield (ca. 4 mg/100 ml erythrocytes) was significantly higher than that of the macroglycolipids from human erythrocytes. The structure of the carbohydrate moiety in the macroglycolipid was analyzed by methylation analysis, Smith degradation, nitrous acid deamination, and chromium trioxide oxidation. Assuming one ceramide residue per molecule, the average number of sugars in the macroglycolipid was about 30. The macroglycolipid had a highly branching structure: Gal(alpha 1 leads to 3)Gal(beta 1 leads to 4)GlcNAc sequences are present at nonreducing termini and leads to 3 Gal(beta 1-4)GlcNAc repeating units are present in the inner core of the sugar chain. Some of the inner galactose residues branch at the C-6 position. Constituents of the ceramide moiety were also characterized.
通过用二碘水杨酸锂萃取并在水苯酚中分配，从兔红细胞膜中制备了一种以半乳糖和N - 乙酰葡糖胺作为主要糖成分的大糖脂，同时还制备了血型糖蛋白。在去污剂Ammonyx LO存在下，通过离子交换色谱法将大糖脂与血型糖蛋白部分有效分离。其产量（约4mg/100ml红细胞）明显高于人红细胞来源的大糖脂。通过甲基化分析、史密斯降解、亚硝酸脱氨和三氧化铬氧化对大糖脂中碳水化合物部分的结构进行了分析。假设每个分子有一个神经酰胺残基，大糖脂中糖的平均数量约为30。大糖脂具有高度分支的结构：在非还原末端存在Gal(α1→3)Gal(β1→4)GlcNAc序列，并且在糖链的内核中存在→3Gal(β1 - 4)GlcNAc重复单元。一些内部半乳糖残基在C - 6位置分支。还对神经酰胺部分的成分进行了表征。
