Clemens L G, Dohanich G P, Witcher J A
J Comp Physiol Psychol. 1981 Oct;95(5):763-70. doi: 10.1037/h0077837.
The ability of cholinergic agents to influence hormone-dependent sexual behavior in female rats was examined. In the first experiment, female sexual behavior, indicated by the incidence of lordosis, was significantly increased in estrogen-treated female rats following bilateral infusion of a cholinergic receptor agonist, carbachol (.5 microgram/cannula), into the medial preoptic area of the brain. Infusion of an artificial cerebrospinal fluid vehicle failed to facilitate lordosis. The incidence of lordosis was normally highest 15 min after carbachol infusion, began to wane by 45 min, and had returned to control levels by 90 min. Further, centrally administered carbachol activated lordosis at lower levels of estrogen priming than did systemically administered progesterone. In a second experiment, female rats, brought into sexual receptivity by administration of estrogen and progesterone, received preoptic infusions of an acetylcholine synthesis inhibitor, hemicholinium-3. Significant reductions in the incidence of lordosis were observed following bilateral infusion of hemicholinium-3 (1.25 microgram/cannula). This inhibition of lordosis was prevented when carbachol (.5 microgram/cannula) was infused along with hemicholinium-3. Results confirm the importance of cholinergic influences on sexual behavior in female rats.
研究了胆碱能药物对雌性大鼠激素依赖性性行为的影响。在第一个实验中，以脊柱前凸发生率为指标的雌性性行为，在双侧向大脑内侧视前区注入胆碱能受体激动剂卡巴胆碱（0.5微克/插管）后，接受雌激素处理的雌性大鼠显著增加。注入人工脑脊液载体未能促进脊柱前凸。脊柱前凸发生率通常在注入卡巴胆碱后15分钟最高，45分钟后开始下降，90分钟后恢复到对照水平。此外，与全身注射孕酮相比，中枢注射卡巴胆碱在较低水平的雌激素预处理下即可激活脊柱前凸。在第二个实验中，通过注射雌激素和孕酮使雌性大鼠进入性接受状态，然后向视前区注入乙酰胆碱合成抑制剂3-羟基-N-甲基氯化胆碱。在双侧注入3-羟基-N-甲基氯化胆碱（1.25微克/插管）后，观察到脊柱前凸发生率显著降低。当与3-羟基-N-甲基氯化胆碱一起注入卡巴胆碱（0.5微克/插管）时，这种对脊柱前凸的抑制作用被阻止。结果证实了胆碱能对雌性大鼠性行为影响的重要性。
