Frentz G, Møller U
J Invest Dermatol. 1981 Dec;77(6):431-3. doi: 10.1111/1523-1747.ep12495660.
A basal cell carcinoma, a Bowenoid carcinoma and clinically normal nonexposed skin from a 76-yr-old male with numerous epidermal tumors on sun-damaged skin were investigated by DNA flow cytometry and the results were compared with data obtained from normal skin of a control group. From each type of skin lesion 3 different biopsies were examined. Each of the DNA frequency distribution histograms showed bimodal configuration revealing no more than one DNA stem line, which was diploid in the unaffected skin and the Bowen carcinoma and hyperdiploid in the basal cell carcinoma. The 3 different types of skin could be clearly separated by means of at least 1 of 3 parameters: S-fraction, G2 + M fraction or S/G2 + M index. The S/G2 + M index came out as the most sensitive discriminator for the tumors. The S-fraction level was higher in the clinically normal skin of the investigated male than in the control group and further high in the tumors.
对一名76岁男性的基底细胞癌、鲍温样癌以及其受阳光损伤皮肤上有众多表皮肿瘤但临床正常的未暴露皮肤进行了DNA流式细胞术研究，并将结果与对照组正常皮肤的数据进行了比较。从每种类型的皮肤病变中选取3个不同的活检样本进行检查。每个DNA频率分布直方图均呈双峰形态，显示不超过一条DNA干系，在未受影响的皮肤和鲍温癌中为二倍体，在基底细胞癌中为超二倍体。通过3个参数中的至少1个：S期分数、G2+M期分数或S/G2+M指数，可以清楚地将3种不同类型的皮肤区分开来。S/G2+M指数是肿瘤最敏感的鉴别指标。被研究男性临床正常皮肤中的S期分数水平高于对照组，在肿瘤中更高。
