Woodland H R, Ballantine J E
J Embryol Exp Morphol. 1980 Dec;60:359-72.
We have studied protein synthesis in the viable hybrid Xenopus laevis (female) X Xenopus borealis (male) using 2D gel electrophoresis. Fourteen borealis-specific proteins were studied. Two of these proteins appeared by the gastrula stage, five in the gastrula and the rest later. Where homologous laevis proteins were tentatively identified, androgenetic haploid hybrids were used to study whether the protein was encoded by stored maternal mRNA, and how long this mRNA persisted. The two proteins appearing in blastulae were probably initially coded by stored maternal mRNA. This was not detectable by the tailbud-tadpole stage, and presumably had been destroyed.
我们利用二维凝胶电泳研究了非洲爪蟾（雌性）与北美爪蟾（雄性）的存活杂交种中的蛋白质合成。研究了14种北美爪蟾特有的蛋白质。其中两种蛋白质在原肠胚阶段出现，五种在原肠胚阶段出现，其余的在之后出现。在初步鉴定出同源的非洲爪蟾蛋白质后，利用雄核发育单倍体杂交种来研究该蛋白质是否由储存的母体mRNA编码，以及这种mRNA持续存在的时间。出现在囊胚中的两种蛋白质可能最初是由储存的母体mRNA编码的。在尾芽-蝌蚪阶段无法检测到这种mRNA，推测它已经被破坏了。
