Neucks S H, Robbins D L, Moore T L, Zuckner J, Dorner R W
J Rheumatol. 1981 Sep-Oct;8(5):811-4.
Synovial fluid (SF) of 16 adult patients with rheumatoid arthritis (RA) and 9 children with juvenile rheumatoid arthritis (JRA) was investigated for the presence of hidden 19S IgM rheumatoid factor (RF). SF was incubated with 500 IU hyaluronidase/g of SF, dialyzed against acetate buffers at decreasing pH, centrifuged and subjected to chromatography on a Sephadex G-200 column. The resulting IgM-containing fraction of SF from 5 seropositive patients (3 adult RA, 2 JRA patients) demonstrated RF activity by the complement-dependent hemolytic assay. Hidden 19S IgM RF was not found in the IgM-containing fractions of SF from 13 adult seronegative RA patients. Hidden 19S IgM RF was shown in the IgM-containing fraction of SF in 4 of 7 children with seronegative JRA.
对16例成年类风湿性关节炎（RA）患者和9例幼年类风湿性关节炎（JRA）患儿的滑液（SF）进行检测，以确定其中是否存在隐匿性19S IgM类风湿因子（RF）。将滑液与每克滑液500 IU的透明质酸酶一起孵育，在pH值逐渐降低的醋酸盐缓冲液中透析，离心，然后在Sephadex G - 200柱上进行层析。5例血清阳性患者（3例成年RA患者，2例JRA患者）的滑液中含IgM的部分通过补体依赖性溶血试验显示出RF活性。13例成年血清阴性RA患者的滑液中含IgM的部分未发现隐匿性19S IgM RF。7例血清阴性JRA患儿中，有4例的滑液中含IgM的部分显示出隐匿性19S IgM RF。
