Kerkis A Iu, Isaeva V V
Ontogenez. 1981;12(6):555-63.
Cell aggregates arising in the suspension culture of the chick embryo myogenic cells were studied by means of electron microscope. The small aggregates are formed by mononuclear myoblasts. The large aggregates are characterized by the tissular organization with the orderly distribution of different cell types. The periphery of such aggregates is occupied by polynuclear myosimplasts in different parts of which individual characters of muscle fiber are expressed to different extent. The cells similar with fibroblasts, chondroblasts, chondrocytes and even fatty cells, as well as collagenous extracellular matrix are situated in the centre of large aggregates. The possibility of origin of different cell types from myoblasts under the specific conditions of suspension culture is discussed.
利用电子显微镜对鸡胚成肌细胞悬浮培养中形成的细胞聚集体进行了研究。小聚集体由单核成肌细胞形成。大聚集体的特征是具有不同细胞类型有序分布的组织样结构。此类聚集体的周边由多核肌单细胞占据,在其不同部位肌纤维的个体特征有不同程度的表达。与成纤维细胞、成软骨细胞、软骨细胞甚至脂肪细胞相似的细胞,以及胶原细胞外基质位于大聚集体的中心。讨论了在悬浮培养的特定条件下成肌细胞产生不同细胞类型的可能性。