家兔离体胃横条儿茶酚胺含量测定

Assay of catecholamines on the isolated transverse stomach strip of the rabbit.

作者信息

Ercan Z S

出版信息

Pharmacology. 1981;23(2):113-5. doi: 10.1159/000137537.

Abstract

Isolated transverse stomach strip of the rabbit was contracted by low concentrations (10(-10) M) of noradrenaline and adrenaline. The contractions were competitively inhibited by phentolamine. Other agonists, such as acetylcholine, histamine, 5-HT, angiotensin II, bradykinin and PGF2 alpha also caused contractions which could be blocked by their specific antagonists. This smooth muscle preparation was more sensitive to noradrenaline and adrenaline than to the other agonists used. Dopamine also produced a contraction when higher concentrations (above 10(-5) M) were used. No tachyphylaxis or spontaneous rhythmicity was observed to repeated application of noradrenaline and adrenaline. It is concluded that rabbit transverse stomach strip could be useful preparation for the bioassay of these catecholamines.

摘要

兔的离体横向胃条被低浓度（10⁻¹⁰ M）的去甲肾上腺素和肾上腺素收缩。这些收缩被酚妥拉明竞争性抑制。其他激动剂，如乙酰胆碱、组胺、5-羟色胺、血管紧张素II、缓激肽和前列腺素F2α也引起收缩，且这些收缩可被其特异性拮抗剂阻断。该平滑肌制剂对去甲肾上腺素和肾上腺素比对所使用的其他激动剂更敏感。当使用较高浓度（高于10⁻⁵ M）的多巴胺时，也会产生收缩。对重复应用去甲肾上腺素和肾上腺素未观察到快速耐受性或自发节律性。结论是兔横向胃条可能是这些儿茶酚胺生物测定的有用制剂。