一种用于大鼠胃条上E型前列腺素生物测定的合理灌流介质。

A rational superfusion medium for the bioassay of E-type prostaglandins on the rat stomach strip.

作者信息

Jager L P, Hofman G A, von Noordwijk J

出版信息

J Pharmacol Methods. 1980 Aug;4(1):19-27. doi: 10.1016/0160-5402(80)90040-6.

Abstract

The optimum composition of a mixture of antagonist to be used in the bioassay of E-type prostaglandins was determined for the rat stomach strip (RSS). In the presence of atropine (10(-7)M), indomethacin (10(-6)M), propranolol (10(-4)M), and tolazoline (10(-4)M) the sensitivity of the RSS to muscarinic, alpha and beta adrenergic and serotonergic agonists was greatly reduced whereas its responsiveness to PGE1 and PGE2 was unaltered. Using the oil-immersion-superfusion technique with this drug mixture, the bioassay of prostaglandins from samples also containing other agonists gave accurate estimates of the PG concentration of the samples using small amounts of 10(-6)M PGE1 or PGE2.

摘要

确定了用于E型前列腺素生物测定的拮抗剂混合物在大鼠胃条（RSS）中的最佳组成。在存在阿托品（10⁻⁷M）、吲哚美辛（10⁻⁶M）、普萘洛尔（10⁻⁴M）和妥拉唑啉（10⁻⁴M）的情况下，RSS对毒蕈碱、α和β肾上腺素能以及5-羟色胺能激动剂的敏感性大大降低，而其对PGE1和PGE2的反应性未改变。使用这种药物混合物的油浸-超灌注技术，对还含有其他激动剂的样品中的前列腺素进行生物测定时，使用少量10⁻⁶M的PGE1或PGE2就能准确估计样品中的PG浓度。