Rogers W R, McCullough B, Caton J E
Toxicology. 1981;20(4):309-21. doi: 10.1016/0300-483x(81)90038-x.
In order to demonstrate quantitatively that cigarette-smoking baboons inhale particulate matter into the lung, a bronchoalveolar lavage method for recovery of [14C]dotriacontane was developed. First, 9 baboons were exposed to a known dose of [14C]dotriacontane labeled particulate matter delivered in a manner providing extensive deposition of particulates in the lung. The lungs of these passively exposed animals then were lavaged so that the efficiency of recovery of the standardized lavage procedure could be determined. Second, 9 baboons actively smoked labeled cigarettes, and the lungs of these animals were lavaged to recover labeled [14C]dotriacontane. The total amount of particulate matter present in the lungs was estimated using the efficiency factor previously determined. The smoking baboons retained an average of 9% of he total cigarette particulate matter. Differences among animals in retention of particulate matter were considerable, and the inter-animal variability was related to differences in number, volume, duration, and pressure of puffs. The retention of particulate matter by baboons is similar to particulate retention by other animal smoke inhalation models.
为了定量证明吸烟的狒狒会将颗粒物吸入肺部，开发了一种用于回收[14C]三十二烷的支气管肺泡灌洗方法。首先，让9只狒狒接触已知剂量的以能使颗粒物在肺部广泛沉积的方式递送的[14C]三十二烷标记颗粒物。然后对这些被动暴露动物的肺部进行灌洗，以便确定标准化灌洗程序的回收效率。其次，让9只狒狒主动吸食标记香烟，并对这些动物的肺部进行灌洗以回收标记的[14C]三十二烷。使用先前确定的效率因子估算肺部存在的颗粒物总量。吸烟的狒狒平均保留了香烟总颗粒物的9%。动物之间在颗粒物保留方面的差异相当大，动物间的变异性与抽吸的次数、体积、持续时间和压力差异有关。狒狒对颗粒物的保留情况与其他动物吸入烟雾模型对颗粒物的保留情况相似。
