Kolb W P, Kolb L M, Wetsel R A, Rogers W R, Shaw J O
Am Rev Respir Dis. 1981 Feb;123(2):226-31. doi: 10.1164/arrd.1981.123.2.226.
The functional activity and antigenic expression of the fifth component of complement (C5) in concentrated bronchoalveolar lavage fluids (BALF) and serum samples obtained from 13 nonsmoking and 13 smoking baboons was quantified by specific hemolytic titration and radioimmunoassays. The number of C5H505 units per milligram of albumin in BALF obtained from nonsmoking and smoking baboons indicated a nearly 2-fold increase in C5 hemolytic activity in lavage fluids as compared to matched serum samples. No significant differences (p greater than 0.05) in C5 hemolytic or antigenic (radioimmunoassay) expression were evident between BALF samples obtained from nonsmoking and smoking baboons. C5 stability in BALF was indicated by the highly significant correlation (p less than 0.005) between C5 hemolytic activity and antigenic expression. The absence of C5-cleaving enzymes in BALF was verified by incubation of 125I-C5 with these fluids, followed by SDS5 polyacrylamide slab gel and autoradiographic analyses. These results demonstrated the presence and stability of substantial quantities of functionally active C5 in the secretions of primate lungs, which represents a readily available source of phlogistic fragments for the initiation of pulmonary inflammation.
通过特异性溶血滴定和放射免疫测定法，对从13只不吸烟和13只吸烟狒狒获得的浓缩支气管肺泡灌洗液（BALF）和血清样本中补体第五成分（C5）的功能活性和抗原表达进行了定量。从不吸烟和吸烟狒狒获得的BALF中每毫克白蛋白的C5H505单位数量表明，与匹配的血清样本相比，灌洗液中C5溶血活性增加了近2倍。从不吸烟和吸烟狒狒获得的BALF样本之间，C5溶血或抗原（放射免疫测定）表达没有显著差异（p大于0.05）。BALF中C5溶血活性与抗原表达之间高度显著的相关性（p小于0.005）表明了C5在BALF中的稳定性。通过将125I-C5与这些液体孵育，然后进行SDS5聚丙烯酰胺平板凝胶和放射自显影分析，验证了BALF中不存在C5裂解酶。这些结果表明，灵长类动物肺部分泌物中存在大量功能活跃的C5，且其具有稳定性，这代表了引发肺部炎症的炎症碎片的现成来源。
