Wells R M, Hill R S, Woodfield D G
Transfusion. 1981 Nov-Dec;21(6):709-14. doi: 10.1046/j.1537-2995.1981.21682085761.x.
Soft and hard-packed red blood cells in four different CPD anticoagulant-preservative solutions were stored with and without added glucose, adenine, and electrolytes. The hemoglobin-oxygen affinity of the red blood cell concentrates was tested over a six-week storage period. No single solution conferred better protection than any other against an expected increase in oxygen affinity due to loss of 2,3-DPG during storage. In all solutions, P50 at pH 7.4 decreased linearly when measured in a physiological system using CO2. After six weeks' storage at 4 C, the normal oxygen-binding properties of red blood cells could be restored in all instances following incubation for one hour in a rejuvenation solution. By contrast, red blood cell ATP levels were highest when resuspending solutions contained adenine and added glucose, but did not significantly compensate the allosteric role of 2,3-DPG in regulating oxygen affinity when the latter became depleted.
将软包装和硬包装的红细胞置于四种不同的CPD抗凝保存液中，分别在添加和不添加葡萄糖、腺嘌呤及电解质的情况下进行储存。在六周的储存期内，对红细胞浓缩液的血红蛋白-氧亲和力进行了测试。对于因储存过程中2,3-二磷酸甘油酸（2,3-DPG）丢失而导致的氧亲和力预期增加，没有一种溶液能比其他溶液提供更好的保护。在所有溶液中，当在使用二氧化碳的生理系统中进行测量时，pH 7.4时的P50呈线性下降。在4℃储存六周后，在复壮溶液中孵育一小时后，所有情况下红细胞的正常氧结合特性均可恢复。相比之下，当重悬液中含有腺嘌呤和添加的葡萄糖时，红细胞ATP水平最高，但当2,3-DPG耗尽时，ATP水平并不能显著补偿其在调节氧亲和力方面的变构作用。
