Stone M, Angus J A
J Pharmacol Exp Ther. 1978 Dec;207(3):705-18.
The standard statistical-computing package GLIM is used to analyze data from agonist-antagonist studies for the estimation of the dissociation constant of the antagonist. The method follows the general approach of D.R. Waud (Analysis of dose-response curves. In Methods in Pharmacology, vol. 3, Smooth Muscle, ed. by E.E. Daniel and M. Paton, Plenum Press, New York, 1975) in employing an iterative nonlinear least-squares curve-fitting procedure. However, it differs in two important respects: 1) it is interactive; and 2) it allows linear adjustments for covariates and experimental design variables. This paper develops develops an alternative useful formulation of departure from simple competitivity and also a graphical display called a "Clark plot" showing how the spacings of the dose-response lines fit the theory of simple competitive antagonism. This plot provides an alternative to the popular Schild plot for which, of necessity, the zero antagonist (control) dose-response line is heavily used both in the experimental design and in the analysis associated with it.
标准统计计算软件包GLIM用于分析激动剂-拮抗剂研究的数据，以估算拮抗剂的解离常数。该方法遵循D.R. 沃德（《剂量反应曲线分析》。载于《药理学方法》第3卷，平滑肌，E.E. 丹尼尔和M. 佩顿编，普伦蒂斯出版社，纽约，1975年）的一般方法，采用迭代非线性最小二乘曲线拟合程序。然而，它在两个重要方面有所不同：1）它是交互式的；2）它允许对协变量和实验设计变量进行线性调整。本文提出了一种偏离简单竞争性的有用替代公式，以及一种名为“克拉克图”的图形显示，展示了剂量反应线的间距如何符合简单竞争性拮抗理论。该图为常用的希尔德图提供了一种替代方案，对于希尔德图，在实验设计及其相关分析中都必然大量使用零拮抗剂（对照）剂量反应线。
