Bencsáth M, Schaff Z, Lapis K, Keresztúry S, Kolláth Z, Hérics M, Hollós I
Acta Morphol Acad Sci Hung. 1981;29(2-3):241-50.
The cytochemical characteristics of the hepatitis B virus (HBV) components, the nuclei and cytoplasm of the infected cells were studied in liver needle biopsy material obtained from patients suffering from serologically HBsAg positive chronic hepatitis. The HBV surface and core components were found to be more resistant to proteolytic enzymes and phospholipase than the other cellular components, and only combined digestion was effective. With preferential RNP and specific DNP staining the accumulation of granules 10-20 nm and 20-40 nm in diameter could be observed in the liver cell nuclei. The cytochemical-morphological polymorphism of the latter was well detectable. Part of the granules corresponded to accumulated perichromatin granules. It is assumed that the groups of granules 20-40 nm in diameter, showing a varying density on DNP staining, similar to the chromatin on RNP staining, and not surrounded by a peripheral area represents one of the forms of appearance of the HB virus core component.
对从血清学检测乙肝表面抗原（HBsAg）阳性的慢性肝炎患者获得的肝穿刺活检材料，研究了乙肝病毒（HBV）成分、被感染细胞的细胞核和细胞质的细胞化学特征。发现HBV表面和核心成分比其他细胞成分对蛋白水解酶和磷脂酶更具抗性，只有联合消化才有效。通过优先的核糖核蛋白（RNP）和特异性脱氧核糖核蛋白（DNP）染色，可在肝细胞核中观察到直径为10 - 20纳米和20 - 40纳米的颗粒积累。后者的细胞化学形态多态性很容易检测到。部分颗粒对应于积累的核周染色质颗粒。据推测，直径为20 - 40纳米的颗粒群，在DNP染色时显示出不同密度，在RNP染色时类似于染色质，且不被周边区域包围，代表了HB病毒核心成分的一种出现形式。
