博来霉素产生的DNA双链断裂和碱不稳定键。
DNA double-strand breaks and alkali-labile bonds produced by bleomycin.
作者信息
Povirk L F, Wübter W, Köhnlein W, Hutchinson F
出版信息
Nucleic Acids Res. 1977 Oct;4(10):3573-80. doi: 10.1093/nar/4.10.3573.
Abstract
Both in linear T2 DNA, analyyzed by velocity sedimentation, and in supercoiled Col EL DNA, analyzed by gel electrophoresis, the number of double-strand breaks produced by bleomycin was directly propotional to the number of single-strand breaks and was far greater than the number expected from random coincidence of single-strand breaks, suggesting that the bleomycin-induced double-strand breaks occur as an independent event. In Col EL DNA, at least twice as many single-strand breaks were found under alkaline assay conditions as were found under neutral conditions, showing the production of alkaline-labile bonds by bleomycin.
摘要
通过速度沉降分析的线性T2 DNA以及通过凝胶电泳分析的超螺旋Col E1 DNA中，博来霉素产生的双链断裂数量与单链断裂数量直接成正比，且远大于单链断裂随机巧合所预期的数量，这表明博来霉素诱导的双链断裂是作为一个独立事件发生的。在Col E1 DNA中，碱性检测条件下发现的单链断裂数量至少是中性条件下的两倍，这表明博来霉素可产生碱不稳定键。
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