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髓磷脂及其亚组分中五种主要糖蛋白的证实。

Demonstration of five major glycoproteins in myelin and myelin subfractions.

作者信息

Mena E E, Moore B W, Hagen S, Agrawal H C

出版信息

Biochem J. 1981 May 1;195(2):525-8. doi: 10.1042/bj1950525.

DOI:10.1042/bj1950525
PMID:7316970
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1162919/
Abstract

Myelin was found to contain five major glycoproteins with molecular weights of 120000, 95000, 88000, 43000 and 38000. Light myelin contained only 5-7% of the amount of these glycoproteins in whole myelin, whereas heavy myelin and the membrane fraction contained amounts nearly identical with whole myelin. Since all the major and minor glycoproteins, with the exception of 120000-mol-wt. glycoprotein, were detected only after treating the myelin membrane with neuraminidase, N-acetylneuraminic acid is a terminal sugar residue in these glycoproteins.

摘要

髓磷脂被发现含有五种主要糖蛋白,分子量分别为120000、95000、88000、43000和38000。轻髓磷脂所含这些糖蛋白的量仅为全髓磷脂的5 - 7%,而重髓磷脂和膜组分所含的量与全髓磷脂几乎相同。由于除了分子量为120000的糖蛋白外,所有主要和次要糖蛋白只有在用神经氨酸酶处理髓磷脂膜后才能检测到,因此N - 乙酰神经氨酸是这些糖蛋白中的末端糖残基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28a9/1162919/21d0ca4dd58a/biochemj00401-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28a9/1162919/21d0ca4dd58a/biochemj00401-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28a9/1162919/21d0ca4dd58a/biochemj00401-0181-a.jpg

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本文引用的文献

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Lectin receptors in central nervous system myelin.中枢神经系统髓鞘中的凝集素受体
J Neurochem. 1980 Jun;34(6):1733-44. doi: 10.1111/j.1471-4159.1980.tb11268.x.
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Protein and glycoprotein composition of myelin subfractions from the developing rat optic nerve and tract.发育中大鼠视神经和视束髓磷脂亚组分的蛋白质和糖蛋白组成
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An improved method of preparing rat brain synaptic membranes. Elimination of a contaminating membrane containing 2',3'-cyclic nucleotide 3'-phosphohydrolase activity.
一种改进的大鼠脑突触膜制备方法。去除含有2',3'-环核苷酸3'-磷酸水解酶活性的污染膜。
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The identity of a myelin-like fraction isolated from developing brain.从发育中的大脑中分离出的一种类髓磷脂组分的特性
Biochem J. 1970 Dec;120(3):635-42. doi: 10.1042/bj1200635.
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A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中氚标记蛋白质和核酸的胶片检测方法。
Eur J Biochem. 1974 Jul 1;46(1):83-8. doi: 10.1111/j.1432-1033.1974.tb03599.x.
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Topographical distribution of complex carbohydrates in the erythrocyte membrane.红细胞膜中复合碳水化合物的拓扑分布。
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Evidence for the close association of a glycoprotein with myelin in rat brain.大鼠脑中一种糖蛋白与髓磷脂紧密关联的证据。
J Neurochem. 1973 Nov;21(5):1177-91. doi: 10.1111/j.1471-4159.1973.tb07573.x.
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Temporal changes in embryonal cell surface recognition.胚胎细胞表面识别的时间变化
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Variation of proteins, enzyme markers and gangliosides in myelin subfractions.髓磷脂亚组分中蛋白质、酶标志物和神经节苷脂的变化
Biochim Biophys Acta. 1973 Dec 5;329(2):305-17. doi: 10.1016/0304-4165(73)90295-x.
10
Myelin subfractions in developing rat brain: characterization and sulphatide metabolism.发育中大鼠脑内的髓磷脂亚组分:特性及硫脂代谢
J Neurochem. 1973 Jun;20(6):1589-603. doi: 10.1111/j.1471-4159.1973.tb00276.x.