Houghton E, Dumasia M C, Wellby J K
Biomed Mass Spectrom. 1981 Nov;8(11):558-64. doi: 10.1002/bms.1200081107.
Negative ion chemical ionization mass spectra of some corticosteroids have been obtained by direct syringe introduction on to the Finnigan moving belt high-performance liquid chromatography-mass spectrometer interface. Proprietary preparations based upon dexamethasone, betamethasone and prednisolone were administered to horses at therapeutic dose level. Urine samples were extracted, the extracts purified by Sephadex LH-20 chromatography and the presence of the parent steroids in the eluates was confirmed by combined high-performance liquid chromatography negative ion chemical ionization mass spectrometry using ammonia as reagent gas.
通过直接用注射器将一些皮质类固醇引入芬尼根移动带高效液相色谱-质谱仪接口，已获得了它们的负离子化学电离质谱。基于地塞米松、倍他米松和泼尼松龙的专利制剂以治疗剂量水平施用于马匹。提取尿液样本，提取物通过葡聚糖凝胶LH - 20色谱法纯化，并用氨作为反应气，通过高效液相色谱-负离子化学电离质谱联用确认洗脱液中母体类固醇的存在。
