Tso W W, Lee C S
Contraception. 1981 Nov;24(5):569-76. doi: 10.1016/0010-7824(81)90060-3.
The concentration effect of gossypol on hypotonically-treated boar spermatozoa with either succinate or pyruvate and malate as substrate was examined. It was found that the pathways involved in the metabolism of succinate was more gossypol-sensitive. The gossypol sensitivity of various electron transport chain (ETC) segments in boar spermatozoa was also investigated with proper choice of electron donors and acceptors. The succinate to cytochrome c segment was the most sensitive one. However this inhibition threshold (1.8 X 10(-5) M gossypol), being higher than either the uncoupling threshold or the concentration threshold at which spermatozoal motility is reduced, suggests that the main target of gossypol antimotility effect does not seem to be the succinate to cytochrome c segment in the ETC.
研究了棉酚对以琥珀酸或丙酮酸与苹果酸为底物的低渗处理公猪精子的浓度效应。结果发现，琥珀酸代谢途径对棉酚更为敏感。还通过适当选择电子供体和受体，对公猪精子中各种电子传递链（ETC）片段的棉酚敏感性进行了研究。琥珀酸到细胞色素c的片段是最敏感的。然而，这种抑制阈值（1.8×10⁻⁵ M棉酚）高于解偶联阈值或精子活力降低时的浓度阈值，这表明棉酚抗活力作用的主要靶点似乎不是ETC中琥珀酸到细胞色素c的片段。
