Mommsen T P, Hochachka P W
Eur J Biochem. 1981 Nov;120(2):345-50. doi: 10.1111/j.1432-1033.1981.tb05710.x.
Mitochondria isolated from systemic hearts of the squid Illex illecebrosus showed high respiratory control ratios, and, with appropriate substrates, the expected ADP/O ratios. Of amino acids tested, proline and ornithine were oxidized at highest rates; of carboxylates, malate, succinate and pyruvate gave the highest state-3 respiration rates. Pyruvate oxidation is enhanced with proline, ornithine, and 1-pyrroline-5-carboxylate (pyrroline carboxylate) all of which can serve to augment the Krebs cycle. However, proline, ornithine and pyrroline carboxylate oxidation is not similarly dependent upon pyruvate. Rotenone inhibited state-3 respiration of malate, proline, ornithine and pyrroline carboxylate. Neither intermediates of fatty acid oxidation nor glycerol 3-phosphate were utilized at significant rates. Key enzymes in proline and ornithine oxidation, i.e. proline dehydrogenase, pyrroline-carboxylate dehydrogenase, ornithine aminotransferase, and glutamate dehydrogenase were located in the mitochondria. The synthesis of proline is catalyzed by pyrroline-carboxylate reductase, which was found exclusively in the cytosol. The respiration, phosphorylation and enzyme data taken together suggest that the main carbon sources for heart mitochondria of Illex are pyruvate plus the proline and ornithine pool.
从乌贼（Illex illecebrosus）的全身心脏中分离出的线粒体显示出高呼吸控制率，并且在使用合适的底物时，呈现出预期的ADP/O比率。在所测试的氨基酸中，脯氨酸和鸟氨酸的氧化速率最高；在羧酸盐中，苹果酸、琥珀酸和丙酮酸产生的状态3呼吸速率最高。丙酮酸氧化会因脯氨酸、鸟氨酸和1-吡咯啉-5-羧酸盐（吡咯啉羧酸盐）而增强，所有这些物质都可以用来增强三羧酸循环。然而，脯氨酸、鸟氨酸和吡咯啉羧酸盐的氧化并不同样依赖于丙酮酸。鱼藤酮抑制了苹果酸、脯氨酸、鸟氨酸和吡咯啉羧酸盐的状态3呼吸。脂肪酸氧化的中间产物和3-磷酸甘油都没有以显著的速率被利用。脯氨酸和鸟氨酸氧化中的关键酶，即脯氨酸脱氢酶、吡咯啉羧酸盐脱氢酶、鸟氨酸转氨酶和谷氨酸脱氢酶都位于线粒体中。脯氨酸的合成由吡咯啉羧酸盐还原酶催化，该酶仅存在于细胞质中。呼吸、磷酸化和酶的数据综合起来表明，乌贼心脏线粒体的主要碳源是丙酮酸加上脯氨酸和鸟氨酸库。
