兔酸性小肠腔灌流液中促胰液素的回收情况。

Recovery of secretin in acid small intestinal lumen perfusates in the rabbit.

作者信息

Lake-Bakaar G, Tovoli S, Li H, Straus E, Yalow R S

出版信息

Horm Metab Res. 1981 Dec;13(12):682-5. doi: 10.1055/s-2007-1019375.

DOI:10.1055/s-2007-1019375

PMID:7319465

Abstract

Immunoreactive secretin concentrations were measured in femoral venous plasma and in intestinal perfusates during acid and neutral perfusion of small intestinal segments in anesthetized rabbits. Steady-state plasma and luminal secretin concentrations were less than 20 pg/ml. Duodenal perfusion with with 0.1 N hydrochloric acid stimulated peak plasma immunoreactive secretin concentrations of 200 - 300 pg/ml within five minutes. Acid stimulated plasma concentrations remained constant during 30 minutes of perfusion. Secretin output into the acid perfusate (2000 - 4000 pg/min) accompanied the release into plasma. Subsequent perfusion with a neutral solution resulted in a rapid return to steady-state plasma and luminal secretin concentrations. Sequential acid perfusion of the ileum, jejunum, and duodenum stimulated similar concentrations of secretin in plasma and luminal perfusates.

摘要

在麻醉兔小肠段进行酸灌注和中性灌注期间，测定股静脉血浆和肠灌流液中免疫反应性促胰液素的浓度。稳态血浆和管腔促胰液素浓度低于20 pg/ml。用0.1 N盐酸十二指肠灌注在5分钟内刺激血浆免疫反应性促胰液素浓度峰值达到200 - 300 pg/ml。在灌注30分钟期间，酸刺激的血浆浓度保持恒定。促胰液素向酸灌流液中的输出量（2000 - 4000 pg/min）伴随着向血浆中的释放。随后用中性溶液灌注导致血浆和管腔促胰液素浓度迅速恢复到稳态。回肠、空肠和十二指肠的顺序酸灌注刺激血浆和管腔灌流液中促胰液素浓度相似。