Sahlberg B L, Axelson M, Collins D J, Sjövall J
J Chromatogr. 1981 Nov 6;217:453-61. doi: 10.1016/s0021-9673(00)88097-8.
A method for separation and analysis of conjugates of ethynylestradiol in urine is described. Steroid conjugates are separated on a lipophilic strong anion exchanger (triethylaminohydroxypropyl Sephadex LH-230), and phenolic steroids released by enzyme hydrolysis or solvolysis ar isolated by chromatography on the same ion exchanger. Steroids carrying an ethynyl group are isolated by chromatography on SP-Sephadex (Ag+). Ethynylestradiol is analyzed by gas chromatography-mass spectrometry of the trimethylsilyl ether, using [9,11,11,12,12-(2)H5] ethynylestradiol and internal standard.
描述了一种尿液中乙炔雌二醇缀合物的分离与分析方法。甾体缀合物在亲脂性强阴离子交换剂（三乙氨基羟丙基葡聚糖凝胶LH - 230）上分离，酶水解或溶剂解释放出的酚类甾体通过在同一离子交换剂上的色谱法分离。带有乙炔基的甾体通过在SP - 葡聚糖凝胶（Ag +）上的色谱法分离。乙炔雌二醇通过三甲基硅醚的气相色谱 - 质谱联用进行分析，使用[9,11,11,12,12 - (2)H5]乙炔雌二醇作为内标。
