Bailey E, Peal J A, Verschoyle R D
J Chromatogr. 1981 Dec 4;219(2):285-90. doi: 10.1016/s0021-9673(00)87938-8.
A method has been developed for the determination of O,S,S-trimethylphosphorodithioate in the plasma, lung, liver, brain and thymus of rats using high-resolution gas chromatography. The organophosphorus compound was extracted from the biological sample with ethyl acetate and analysed on a carbowax 20M fused-silica capillary column with a nitrogen-phosphorus specific detector. O,S,S-Triethylphosphorodithioate was used as an internal standard added to the sample before extraction. The sensitivity of the method allowed the compound to be measured in 0.1-ml aliquots of plasma or in 20-mg wet weight of tissue down to a level of 5 ng/sample. The method has been applied to a pharmacokinetic study in the rat after an oral or intravenous dosage with 25 mg/kg of O,S,S-trimethylphosphorodithioate.
已开发出一种使用高分辨率气相色谱法测定大鼠血浆、肺、肝、脑和胸腺中O,S,S-三甲基二硫代磷酸酯的方法。用乙酸乙酯从生物样品中提取有机磷化合物，并在涂有聚乙二醇20M的熔融石英毛细管柱上进行分析，使用氮磷特异性检测器。在提取前向样品中加入O,S,S-三乙基二硫代磷酸酯作为内标。该方法的灵敏度使得能够在0.1 ml血浆等分试样或20 mg湿重组织中检测该化合物，最低检测水平为5 ng/样品。该方法已应用于大鼠口服或静脉注射25 mg/kg O,S,S-三甲基二硫代磷酸酯后的药代动力学研究。
