Andriollo O, Lartigue-Mattei C, Chabard J L, Bargnoux H, Petit J, Berger J A, Pognat J F
Groupe de Recherches en Biodynamique du Médicament, Faculté de Pharmacie, Clermont-Ferrand, France.
J Chromatogr. 1992 Mar 27;575(2):301-5. doi: 10.1016/0378-4347(92)80161-i.
A specific and highly sensitive method for the measurement of trazodone in plasma and brain of rat is presented. The compound and the internal standard were extracted from alkalinized samples with hexane and analysed by capillary gas chromatography with nitrogen-selective detection. The method was demonstrated to be accurate and precise. The limits of determination were 2 ng/ml for plasma and 24 ng/g for brain, which makes this procedure suitable for pharmacokinetic analysis.
本文介绍了一种测定大鼠血浆和脑中曲唑酮的特异性高灵敏度方法。该化合物和内标从碱化样品中用己烷萃取，并用氮选择性检测的毛细管气相色谱法进行分析。该方法经证明准确且精密。血浆的测定限为2 ng/ml，脑的测定限为24 ng/g，这使得该方法适用于药代动力学分析。
