Goto M, Nakamura T, Ishii D
J Chromatogr. 1981 Nov 13;226(1):33-42. doi: 10.1016/s0378-4347(00)84203-6.
A new micro high-performance liquid chromatographic system has been developed, which determines catecholamines from directly injected human urine without any sample pretreatment. The system consists of a mixing junction for adjusting the sample to pH 8.5, a micro alumina precolumn for enriching catecholamines in the sample, and a dual electrochemical detector for selectively detecting catecholamines based on their electrochemical reversibility. The system is able to operate with directly injected body fluids and to determine simultaneously all of the four catecholamines with high precision.
一种新型的微型高效液相色谱系统已被开发出来，该系统无需对样品进行任何预处理就能直接从注入的人体尿液中测定儿茶酚胺。该系统由一个用于将样品调节至pH 8.5的混合接头、一个用于富集样品中儿茶酚胺的微型氧化铝预柱以及一个基于儿茶酚胺的电化学可逆性选择性检测儿茶酚胺的双电化学检测器组成。该系统能够直接对注入的体液进行检测，并能高精度地同时测定所有四种儿茶酚胺。
