Hjemdahl P, Larsson P T, Bradley T, Akerstedt T, Anderzén I, Sigurdsson K, Gillberg M, Lundberg U
Department of Clinical Pharmacology, Karolinska Hospital, Stockholm, Sweden.
J Chromatogr. 1989 Sep 29;494:53-66. doi: 10.1016/s0378-4347(00)82656-0.
In order to validate different methods of measuring urinary catecholamines (norepinephrine, epinephrine and dopamine) in humans, methods based on separation of catecholamines using reversed-phase or cation-exchange high-performance liquid chromatography with electrochemical detection were compared with an autoanalyser-based fluorescence method. Different methods for pre-chromatography sample purification were also studied. For measurements of urinary catecholamines, the reversed-phase-based chromatographic techniques studied were found to give less reliable results than cation-exchange chromatography, even if one of them (Clin Rep Urine Catecholamine Kit) gave almost as precise estimates. The autoanalyser technique yielded good results. It is concluded that cation-exchange chromatography with an appropriate sample work-up procedure (a combination of organic solvent extraction and alumina adsorption) is a reliable and accurate method for analyses of urinary catecholamines.
为了验证测量人体尿儿茶酚胺（去甲肾上腺素、肾上腺素和多巴胺）的不同方法，将基于反相或阳离子交换高效液相色谱分离儿茶酚胺并结合电化学检测的方法与基于自动分析仪的荧光法进行了比较。还研究了色谱分离前样品净化的不同方法。对于尿儿茶酚胺的测量，发现所研究的基于反相的色谱技术比阳离子交换色谱法给出的结果可靠性更低，即使其中一种方法（临床报告尿儿茶酚胺试剂盒）给出的估计几乎同样精确。自动分析仪技术产生了良好的结果。结论是，采用适当的样品处理程序（有机溶剂萃取和氧化铝吸附相结合）的阳离子交换色谱法是分析尿儿茶酚胺的可靠且准确的方法。
