使用混合模式柱和八通道电化学检测器通过高效液相色谱法同时测定尿液中的儿茶酚胺、其主要代谢物和血清素。

Simultaneous determination of catecholamines, their basic metabolites and serotonin in urine by high-performance liquid chromatography using a mixed-mode column and an eight-channel electrochemical detector.

作者信息

Mashige F, Matsushima Y, Miyata C, Yamada R, Kanazawa H, Sakuma I, Takai N, Shinozuka N, Ohkubo A, Nakahara K

机构信息

Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Biomed Chromatogr. 1995 Sep-Oct;9(5):221-5. doi: 10.1002/bmc.1130090506.

DOI:10.1002/bmc.1130090506

PMID:8593422

Abstract

A high-performance liquid chromatographic method for simultaneous determination of free catecholamines, their basic metabolites and serotonin in human urine was developed. The compounds were separated on a precolumn of cation-exchange resin and a mixed-mode (C18/cation-exchange) column and determined by an eight-channel electrochemical detector. By this method, free norepinephrine (NE), epinephrine (E), dopamine (DA), normetanephrine (NMN), metanephrine (MN), 3-methoxy-tyramine (3-MT) and serotonin (5-HT) were determined in 40 min with inter-assay precision of 1.9, 3.2, 1.2, 1.6, 1.6, 5.5 and 3.2%, respectively. Detection limits were between 0.05 (E) and 0.3 (3-MT) pmol. The mean values of analytical recovery for NE, E, DA, NMN, MN, 3-MT, 5-HT were 99, 104, 104, 99, 102, 109 and 99%, respectively.

摘要

建立了一种同时测定人尿中游离儿茶酚胺、其碱性代谢产物和5-羟色胺的高效液相色谱法。这些化合物在阳离子交换树脂预柱和混合模式（C18/阳离子交换）柱上分离，并用八通道电化学检测器进行测定。通过该方法，在40分钟内测定了游离去甲肾上腺素（NE）、肾上腺素（E）、多巴胺（DA）、去甲变肾上腺素（NMN）、变肾上腺素（MN）、3-甲氧基酪胺（3-MT）和5-羟色胺（5-HT），批间精密度分别为1.9%、3.2%、1.2%、1.6%、1.6%、5.5%和3.2%。检测限在0.05（E）至0.3（3-MT）pmol之间。NE、E、DA、NMN、MN、3-MT、5-HT的分析回收率平均值分别为99%、104%、1（此处原文有误，应为104）、99%、102%、109%和99%。