Mashige F, Matsushima Y, Miyata C, Yamada R, Kanazawa H, Sakuma I, Takai N, Shinozuka N, Ohkubo A, Nakahara K
Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biomed Chromatogr. 1995 Sep-Oct;9(5):221-5. doi: 10.1002/bmc.1130090506.
A high-performance liquid chromatographic method for simultaneous determination of free catecholamines, their basic metabolites and serotonin in human urine was developed. The compounds were separated on a precolumn of cation-exchange resin and a mixed-mode (C18/cation-exchange) column and determined by an eight-channel electrochemical detector. By this method, free norepinephrine (NE), epinephrine (E), dopamine (DA), normetanephrine (NMN), metanephrine (MN), 3-methoxy-tyramine (3-MT) and serotonin (5-HT) were determined in 40 min with inter-assay precision of 1.9, 3.2, 1.2, 1.6, 1.6, 5.5 and 3.2%, respectively. Detection limits were between 0.05 (E) and 0.3 (3-MT) pmol. The mean values of analytical recovery for NE, E, DA, NMN, MN, 3-MT, 5-HT were 99, 104, 104, 99, 102, 109 and 99%, respectively.
建立了一种同时测定人尿中游离儿茶酚胺、其碱性代谢产物和5-羟色胺的高效液相色谱法。这些化合物在阳离子交换树脂预柱和混合模式（C18/阳离子交换）柱上分离，并用八通道电化学检测器进行测定。通过该方法，在40分钟内测定了游离去甲肾上腺素（NE）、肾上腺素（E）、多巴胺（DA）、去甲变肾上腺素（NMN）、变肾上腺素（MN）、3-甲氧基酪胺（3-MT）和5-羟色胺（5-HT），批间精密度分别为1.9%、3.2%、1.2%、1.6%、1.6%、5.5%和3.2%。检测限在0.05（E）至0.3（3-MT）pmol之间。NE、E、DA、NMN、MN、3-MT、5-HT的分析回收率平均值分别为99%、104%、1（此处原文有误，应为104）、99%、102%、109%和99%。
