Hay I D, Annesley T M, Jiang N S, Gorman C A
J Chromatogr. 1981 Dec 11;226(2):383-90. doi: 10.1016/s0378-4347(00)86072-7.
A method for the determination of D- and L-thyroxine in human serum is described. The method involves extraction of thyroxine from serum and the separation of thyroxine enantiomers on a reversed-phase, high-performance liquid chromatographic column by use of a chiral eluent containing L-proline and cupric sulfate. Satisfactory resolution of the enantiomers of thyroxine, triiodothyronine, and reverse triiodothyronine can be achieved in 12 min and, employing amperometric detection to monitor the separation, the detection limit for serum thyroxine is in the range of 1--3 ng per injected sample.
本文描述了一种测定人血清中 D- 和 L-甲状腺素的方法。该方法包括从血清中提取甲状腺素，并使用含有 L-脯氨酸和硫酸铜的手性洗脱剂在反相高效液相色谱柱上分离甲状腺素对映体。甲状腺素、三碘甲状腺原氨酸和反三碘甲状腺原氨酸的对映体在 12 分钟内可实现满意的分离，采用安培检测监测分离过程，血清甲状腺素的检测限为每进样一次 1 - 3 ng。
