Petersen B A, Giese R W, Larsen P R, Karger B L
Clin Chem. 1977 Aug;23(8):1389-96.
We describe a simultaneous, absolute analysis for free (dialyzable) triiodothyronine and thyroxine in serum by the sequence: dialysis, internal standard addition to the dialysate, cation-exchange chromatography, derivatization, and gas chromatography with electron capture detection. The detection limit is 0.2 pg and the precision (repeatability) in the range of 4 to 20 ng/liter is 1.6%. Mean values for free T3 and T4 in 29 euthryoid sera were 4.2 and 18.9 ng/liter, respectively, in close agreement with accepted values. Correlation coefficients for the two compounds were 0.99 and 0.97, respectively, when this procedure was compared with a method involving tracer dialysis and radioimmunoassay. A chromatographic peak was seen for free 3,3',5'-triiodothyronine in the analyses of two of eight hyperthyroid sera, and, with a larger sample volume, from a euthyroid serum. The new method is a potential reference and research procedure for analysis of free iodothyronines.
我们描述了一种通过以下步骤对血清中游离（可透析）三碘甲状腺原氨酸和甲状腺素进行同步绝对分析的方法：透析、向透析液中添加内标、阳离子交换色谱法、衍生化以及带电子捕获检测的气相色谱法。检测限为0.2皮克，在4至20纳克/升范围内的精密度（重复性）为1.6%。29份甲状腺功能正常血清中游离T3和T4的平均值分别为4.2和18.9纳克/升，与公认值非常一致。当将此方法与涉及示踪剂透析和放射免疫分析的方法进行比较时，这两种化合物的相关系数分别为0.99和0.97。在8份甲状腺功能亢进血清中的2份分析以及使用更大样本量时，从一份甲状腺功能正常血清中均检测到了游离3,3',5'-三碘甲状腺原氨酸的色谱峰。该新方法是分析游离碘甲状腺原氨酸的一种潜在参考和研究方法。
