Walker R A
J Pathol. 1981 Nov;135(3):237-47. doi: 10.1002/path.1711350308.
A cytochemical method has been used to demonstrate oestrogen receptor in histological sections of breast carcinomas, and has given similar qualitative results to the biochemical dextra-coated charcoal method. Two conjugation techniques were employed to couple horseradish peroxidase to 17 beta-oestradiol-6-O-carboxymethyloxime-bovine serum albumin. The periodate conjugation method led to a greater degree of coupling but a higher concentration was required for demonstration of oestrogen receptor than that needed when the two-stage glutaraldehyde conjugation method was use, even though this resulted in a lesser degree of coupling. Care in the preparation of tissue has been found to be essential. Formalin-fixed, paraffin-embedded tissue was unsuitable; rapid freezing of fresh tissue, only brief air-drying or acetone fixation of tissue sections and short-term storage were important. A correlation has been shown between the presence of oestrogen receptor in breast carcinomas and good histological differentiation. The poorer differentiated tumours were also found to contain fewer reactive cells.
一种细胞化学方法已用于在乳腺癌组织切片中显示雌激素受体，并且其定性结果与生化右旋糖包被活性炭法相似。采用了两种偶联技术将辣根过氧化物酶与17β-雌二醇-6-O-羧甲基肟-牛血清白蛋白偶联。高碘酸盐偶联法导致更高程度的偶联，但与使用两步戊二醛偶联法时相比，显示雌激素受体需要更高的浓度，尽管后者导致的偶联程度较低。已发现组织制备过程中的小心操作至关重要。福尔马林固定、石蜡包埋的组织不合适；新鲜组织的快速冷冻、组织切片仅短暂风干或丙酮固定以及短期保存很重要。已显示乳腺癌中雌激素受体的存在与良好的组织学分化之间存在相关性。还发现分化较差的肿瘤含有较少的反应性细胞。
