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钠梯度调控对豚鼠结肠带细胞钙、45Ca通量及细胞钠的影响

Effects of sodium gradient manipulation upon cellular calcium, 45Ca fluxes and cellular sodium in the guinea-pig taenia coli.

作者信息

Aaronson P, van Breemen C

出版信息

J Physiol. 1981;319:443-61. doi: 10.1113/jphysiol.1981.sp013920.

DOI:10.1113/jphysiol.1981.sp013920
PMID:7320922
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1243850/
Abstract
  1. Sucrose and choline were utilized as NaCl substitutes in order to investigate Na-Ca interactions in the smooth muscle of the guinea-pig taenia coli.2. Progressive substitution of NaCl by sucrose caused a progressive increase in cellular exchangeable Ca. This uptake, which amounted to about 300 mumole Ca/kg tissue upon total Na replacement, reached a plateau within 20 min. Complete substitution of NaCl by choline chloride caused cellular Ca to increase rapidly to an initial peak, and then decrease to a stable plateau which was also about 300 mumole/kg above control.3. Replacement of NaCl by either sucrose or choline chloride caused a transient increase in the Ca influx rate, which was measured using a 3 min pulse labelling with (45)Ca. This increase was more pronounced in choline chloride.4. NaCl substitution by either sucrose or choline chloride caused a decrease in the (45)Ca efflux rate. Two exponential components of transmembrane (45)Ca efflux were found in control and Na-free media.5. Treatment of tissues with 3 x 10(-5)m-ouabain did not significantly affect the cellular Ca content after 80 min, at which time the Na and K gradients were largely dissipated.6. Removal of medium K caused a slower dissipation of the Na and K gradients. This treatment decreased cellular Ca, did not affect the Ca influx rate, and increased the (45)Ca efflux rate.7. Tissues were incubated in depolarizing media containing 10(-4)m-ouabain in order to remove the Na gradient. Subsequent measurement of cellular Na indicated the absence of a significant fraction of bound Na.8. The ratio Na/Na had a value of 6.3 in control medium, and decreased as Na was progressively lowered by sucrose substitution, reaching a value of < 1 in a medium containing 5 mm-Na.9. These experiments provide evidence that a Na-Ca exchange carrier does not play an important role in regulation of tension in this muscle, and also indicate that the Ca gradient is not solely dependent on the Na gradient in guinea-pig taenia coli.
摘要

  1. 为了研究豚鼠结肠带平滑肌中的钠钙相互作用，使用蔗糖和胆碱作为氯化钠的替代物。

  2. 用蔗糖逐步替代氯化钠导致细胞可交换钙逐渐增加。在完全替代钠后，这种摄取量达到约300微摩尔钙/千克组织，在20分钟内达到平稳状态。用氯化胆碱完全替代氯化钠导致细胞钙迅速增加至初始峰值，然后降至稳定的平稳状态，该平稳状态也比对照高约300微摩尔/千克。

  3. 用蔗糖或氯化胆碱替代氯化钠导致钙流入速率短暂增加，这是通过用（45）钙进行3分钟脉冲标记来测量的。这种增加在氯化胆碱中更明显。

  4. 用蔗糖或氯化胆碱替代氯化钠导致（45）钙流出速率降低。在对照和无钠培养基中发现了跨膜（45）钙流出的两个指数成分。

  5. 用3×10（-5）米哇巴因处理组织80分钟后，细胞钙含量没有明显影响，此时钠和钾梯度基本消失。

  6. 去除培养基中的钾导致钠和钾梯度消散较慢。这种处理降低了细胞钙，不影响钙流入速率，并增加了（45）钙流出速率。

  7. 将组织在含有10（-4）米哇巴因的去极化培养基中孵育以消除钠梯度。随后对细胞钠的测量表明不存在大量结合钠。

  8. 在对照培养基中，[钠]（外）/[钠]（内）的比值为6.3，随着用蔗糖替代使[钠]（外）逐渐降低，该比值在含有5毫米钠的培养基中降至<1。

  9. 这些实验提供了证据，表明钠钙交换载体在调节该肌肉张力中不发挥重要作用，并且还表明在豚鼠结肠带中钙梯度并非仅依赖于钠梯度。

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J Physiol. 1981;319:443-61. doi: 10.1113/jphysiol.1981.sp013920.
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本文引用的文献

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Sodium gradient, smooth muscle tone, and blood pressure regulation.钠梯度、平滑肌张力与血压调节。
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Membrane potentials recorded with high-resistance micro-electrodes; and the effects of changes in ionic environment on the electrical and mechanical activity of the smooth muscle of the taenia coli of the guineapig.用高电阻微电极记录的膜电位；以及离子环境变化对豚鼠结肠带平滑肌电活动和机械活动的影响。
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The action of ouabain on the smooth muscle cells of the guinea-pig's taenia coli.哇巴因对豚鼠结肠带平滑肌细胞的作用。
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The effect of sodium and calcium on the action potential of the smooth muscle of the guinea-pig taenia coli.钠和钙对豚鼠结肠带平滑肌动作电位的影响。
J Physiol. 1969 Feb;200(3):637-54. doi: 10.1113/jphysiol.1969.sp008713.
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The dependence of calcium efflux from cardiac muscle on temperature and external ion composition.心肌中钙外流对温度和细胞外离子成分的依赖性。
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Calculation of the membrane potential in smooth muscle cells of the guinea-pig's taenia coli by the Goldman equation.用戈德曼方程计算豚鼠结肠带平滑肌细胞的膜电位。
J Physiol. 1969 Nov;205(1):193-208. doi: 10.1113/jphysiol.1969.sp008960.
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Influences of sodium and calcium on the recovery process from potassium contracture in the guinea-pig taenia coli.钠和钙对豚鼠结肠带钾挛缩恢复过程的影响。
J Physiol. 1972 Jul;224(2):489-500. doi: 10.1113/jphysiol.1972.sp009907.