长春碱和秋水仙碱对体内及体外肾素分泌的影响。

The influence of vinblastine and colchicine on renin secretion in vivo and in vitro.

作者信息

Hackenthal E, Schwertschlag U, Hackenthal R

出版信息

Klin Wochenschr. 1978;56 Suppl 1:61-6. doi: 10.1007/BF01477454.

Abstract

The effect of the microtubule inhibitors colchicine and vinblastine on renin release in vivo and in vitro was studied. Injection of 0.5 mg/100 g i.v. of colchicine or vinblastine to furosemide treated rats on a low salt diet resulted in a decrease of plasma renin as well as plasma angiotensinogen concentration during a 5 h observation period. Renin release from rat kidney slices was diminished by vinblastine (5 x 10(-5) M), when basal or stimulated (by isobutylmethylxanthine and isoproterenol) renin release was measured. Colchicine at 5 x 10(-5) M had no effect under these conditions. Renin release from the isolated perfused rat kidney was increased 2--3 fold by vinblastine (10(-5) M) or colchicine (10(-4) M). The maximal response of renin release to isoproterenol (10(-7) M) was not changed when vinblastine (10(-5) M) or colchicine (10(-4) M) were present in the perfusion medium. The contrasting results cast considerable doubts on the suitability of microtubule inhibitors in studies on renin secretion.

摘要

研究了微管抑制剂秋水仙碱和长春碱对体内及体外肾素释放的影响。给低盐饮食且用速尿处理的大鼠静脉注射0.5mg/100g秋水仙碱或长春碱，在5小时观察期内，血浆肾素以及血浆血管紧张素原浓度均降低。当测定基础或刺激（用异丁基甲基黄嘌呤和异丙肾上腺素）的肾素释放时，长春碱（5×10⁻⁵M）可减少大鼠肾切片的肾素释放。在这些条件下，5×10⁻⁵M的秋水仙碱无作用。长春碱（10⁻⁵M）或秋水仙碱（10⁻⁴M）可使离体灌注大鼠肾脏的肾素释放增加2至3倍。当灌注液中存在长春碱（10⁻⁵M）或秋水仙碱（10⁻⁴M）时，肾素对异丙肾上腺素（10⁻⁷M）的最大反应未改变。这些相互矛盾的结果使人对微管抑制剂在肾素分泌研究中的适用性产生了很大怀疑。