Tonelli Q, Meints R H
J Supramol Struct. 1978;8(1):67-78. doi: 10.1002/jss.400080106.
Vibrio cholerae neuraminidase (VCN) treatment of donor bone marrow cells results in a reduction in the number of hematopoietic colonies (CFUs) formed in the spleens of lethally irradiated mice. Treatment of marrow cells with sodium periodate under mild conditions, known to preferentially oxidize sialic acid, also reduced CFUs while subsequent potassium borohydride reduction restored CFUs to 80% of control levels. Innoculum viability as measured by in vitro incorporation of tritiated precursors into proteins, nucleic acids, and oligosaccharides was unaffected by VCN treatment. The ability of bone marrow cells in culture to respond to the hormone erythropoietin, as measured by the incorporation of 59Fe into cyclohexanone-extractable heme, was also not affected by neuraminidase, making a cytotoxic effect of the VCN preparation unlikely. Incubation of VCN-treated marrow with either beta-galactosidase or trypsin had no effect on the VCN-induced reduction in CFUs. These results are consistent with the idea that membrane sialic acid plays a direct and specific role in the implantation and development of CFUs.
用霍乱弧菌神经氨酸酶（VCN）处理供体骨髓细胞会导致在接受致死剂量照射的小鼠脾脏中形成的造血集落（CFU）数量减少。在温和条件下用过碘酸钠处理骨髓细胞，已知过碘酸钠会优先氧化唾液酸，这也会减少CFU，而随后用硼氢化钾还原可使CFU恢复到对照水平的80%。通过体外将氚标记的前体掺入蛋白质、核酸和寡糖来测定的接种物活力不受VCN处理的影响。通过将59Fe掺入环己酮可提取的血红素来测定，培养的骨髓细胞对促红细胞生成素的反应能力也不受神经氨酸酶的影响，这使得VCN制剂不太可能具有细胞毒性作用。用β-半乳糖苷酶或胰蛋白酶孵育经VCN处理的骨髓对VCN诱导的CFU减少没有影响。这些结果与细胞膜唾液酸在CFU的植入和发育中起直接和特定作用的观点一致。
