Gilbert S F, Axelman J, Migeon B R
Am J Hum Genet. 1981 Nov;33(6):950-6.
The heterogeneity of cell morphology characteristics of some colonies of human fetal kidney and amniotic fluid cells has been analyzed by biochemical and cell-cloning techniques. All the presumed subclones derived from dimorphic colonies were initially epithelioid, but some cells became fibroblastlike as the cell density increased. To determine if the observed heterogeneity occurred within clonal populations of cells, we determined the isozyme phenotype of dimers from renal cells heterozygous for glucose-6-phosphate dehydrogenase (G6PD). Colonies showing mixed cellular morphology expressed only a single G6PD isozyme, thus revealing their single-cell origin. Our results indicate that cell morphology is influenced by the cellular density within the clone, and that a single human renal cell in vitro can yield progeny of two morphological types.
已通过生化和细胞克隆技术分析了人胎儿肾和羊水细胞某些集落的细胞形态特征的异质性。所有推测来自双态集落的亚克隆最初都是上皮样的，但随着细胞密度增加，一些细胞变成了成纤维细胞样。为了确定观察到的异质性是否发生在细胞的克隆群体中，我们测定了葡萄糖-6-磷酸脱氢酶（G6PD）杂合的肾细胞二聚体的同工酶表型。显示混合细胞形态的集落仅表达单一的G6PD同工酶，从而揭示了它们的单细胞起源。我们的结果表明，细胞形态受克隆内细胞密度的影响，并且体外单个人类肾细胞可以产生两种形态类型的后代。
