• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

正常人肝脏克隆培养物中白蛋白及其他血清蛋白的产生

Production of albumin and other serum proteins by clonal cultures of normal human liver.

作者信息

Kaighn M E, Prince A M

出版信息

Proc Natl Acad Sci U S A. 1971 Oct;68(10):2396-400. doi: 10.1073/pnas.68.10.2396.

DOI:10.1073/pnas.68.10.2396
PMID:5002818
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC389430/
Abstract

Clonal cell strains have been isolated from normal human liver. These cells, while resembling fibroblasts morphologically, function as hepatocytes, as shown by their ability to synthesize and secrete an antigen identical to human serum albumin. Human diploid and aneuploid cell lines from nonliver sources do not exhibit this property. The spectrum of serum proteins synthesized varied from clone to clone and cell line to cell line.

摘要

已从正常人肝脏中分离出克隆细胞株。这些细胞在形态上类似于成纤维细胞，但却具有肝细胞的功能，这体现在它们能够合成并分泌一种与人血清白蛋白相同的抗原。来自非肝脏来源的人二倍体细胞系和非整倍体细胞系则不具备此特性。不同克隆之间以及不同细胞系之间合成的血清蛋白谱各不相同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/61a661aaad18/pnas00085-0086-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/18d9dbfa1701/pnas00085-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/c46b25cf9116/pnas00085-0084-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/21cd7148a6dd/pnas00085-0084-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/2ce419bf4469/pnas00085-0084-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/259c592dc940/pnas00085-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/61a661aaad18/pnas00085-0086-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/18d9dbfa1701/pnas00085-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/c46b25cf9116/pnas00085-0084-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/21cd7148a6dd/pnas00085-0084-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/2ce419bf4469/pnas00085-0084-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/259c592dc940/pnas00085-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d58b/389430/61a661aaad18/pnas00085-0086-a.jpg

相似文献

1
Production of albumin and other serum proteins by clonal cultures of normal human liver.正常人肝脏克隆培养物中白蛋白及其他血清蛋白的产生
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2396-400. doi: 10.1073/pnas.68.10.2396.
2
Production of serum proteins by primary cultures of adult human liver.
Biomedicine. 1973 May;18(3):248-55.
3
Development of gamma G, gamma A, gamma M, beta IC-beta IA, C 1 esterase inhibitor, ceruloplasmin, transferrin, hemopexin, haptoglobin, fibrinogen, plasminogen, alpha 1-antitrypsin, orosomucoid, beta-lipoprotein, alpha 2-macroglobulin, and prealbumin in the human conceptus.人类胚胎中γG、γA、γM、βIC-βIA、C1酯酶抑制剂、铜蓝蛋白、转铁蛋白、血红素结合蛋白、触珠蛋白、纤维蛋白原、纤溶酶原、α1-抗胰蛋白酶、类粘蛋白、β-脂蛋白、α2-巨球蛋白和前白蛋白的发育情况。
J Clin Invest. 1969 Aug;48(8):1433-46. doi: 10.1172/JCI106109.
4
Production of plasma proteins by subcultures of adult human liver.
Biomedicine. 1973 Aug 10;19(8):361-4.
5
[Synthesis of plasma proteins by cancerous and normal human hepatocytes in culture].[培养的人癌性和正常肝细胞合成血浆蛋白]
Pathol Biol (Paris). 1974 Dec;22(10):867-76.
6
Serum protein synthesis by somatic cell hybrids.体细胞杂种的血清蛋白合成
Somatic Cell Genet. 1982 Jul;8(4):433-49. doi: 10.1007/BF01538706.
7
Plasma protein synthesis by the isolated perfused regenerating rat liver.离体灌注的再生大鼠肝脏合成血浆蛋白的研究
Biochim Biophys Acta. 1966 Dec 28;130(2):486-92. doi: 10.1016/0304-4165(66)90244-3.
8
Serum protein changes in the parturient mother, fetus and newborn infant.产妇、胎儿及新生儿的血清蛋白变化
J Obstet Gynaecol Br Commonw. 1970 Jun;77(6):511-7. doi: 10.1111/j.1471-0528.1970.tb03558.x.
9
Effects on net plasma protein synthesis of removal of L-tryptophan or L-threonine from a complete amino acid mixture: studies in the isolated perfused rat liver.从完整氨基酸混合物中去除L-色氨酸或L-苏氨酸对血浆净蛋白合成的影响：在离体灌注大鼠肝脏中的研究
Am J Clin Nutr. 1971 Jun;24(6):718-29. doi: 10.1093/ajcn/24.6.718.
10
Effects of hypophysectomy of liver donor on net synthesis of specific plasma proteins by the isolated perfused rat liver. Modulation of synthesis of albumin, fibrinogen, alpha 1-acid glycoprotein, alpha 2-(acute phase)-globulin, and haptoglobin by insulin, cortisol, triiodothyronine, and growth hormone.肝供体垂体切除对离体灌注大鼠肝脏特定血浆蛋白净合成的影响。胰岛素、皮质醇、三碘甲状腺原氨酸和生长激素对白蛋白、纤维蛋白原、α1-酸性糖蛋白、α2-（急性期）球蛋白和触珠蛋白合成的调节。
J Biol Chem. 1974 Aug 25;249(16):5062-9.

引用本文的文献

1
Proteomic Characterization of Annexin l (ANX1) and Heat Shock Protein 27 (HSP27) as Biomarkers for Invasive Hepatocellular Carcinoma Cells.膜联蛋白l（ANX1）和热休克蛋白27（HSP27）作为侵袭性肝癌细胞生物标志物的蛋白质组学特征分析
PLoS One. 2015 Oct 2;10(10):e0139232. doi: 10.1371/journal.pone.0139232. eCollection 2015.
2
Cell lines from non-neoplastic liver and hepatocellular carcinoma tissue from a single patient.
In Vitro Cell Dev Biol Anim. 1996 Mar;32(3):135-7. doi: 10.1007/BF02723678.
3
Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens.猿猴病毒40大T抗原永生化的正常人肝上皮细胞表达肝细胞特征并代谢化学致癌物。

本文引用的文献

1
TISSUE CULTURE POPULATIONS AND THEIR RELATION TO THE TISSUE OF ORIGIN.组织培养群体及其与起源组织的关系。
Proc Natl Acad Sci U S A. 1960 Jul;46(7):963-72. doi: 10.1073/pnas.46.7.963.
2
The growth in vitro of massive cultures of liver cells.肝细胞大规模培养的体外生长
J Natl Cancer Inst. 1952 Jun;12(6):1245-65.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5123-7. doi: 10.1073/pnas.90.11.5123.
4
The establishment and continuous subculturing of normal human adult hepatocytes: expression of differentiated liver functions.
Cell Biol Toxicol. 1993 Oct-Dec;9(4):385-403. doi: 10.1007/BF00754467.
5
Phenotypic heterogeneity within clones of fetal human cells.人胎儿细胞克隆内的表型异质性。
Am J Hum Genet. 1981 Nov;33(6):950-6.
6
Isolation and primary culture of adult human hepatocytes. Ultrastructural and functional studies.成人肝细胞的分离与原代培养。超微结构与功能研究。
Cell Tissue Res. 1981;218(1):13-21. doi: 10.1007/BF00210087.
7
Preparation of human hepatocytes for primary culture.用于原代培养的人肝细胞的制备。
In Vitro. 1982 May;18(5):483-91. doi: 10.1007/BF02796477.
8
Induction of plasma protein secretion in a newly established human hepatoma cell line.在一种新建立的人肝癌细胞系中诱导血浆蛋白分泌。
Mol Cell Biol. 1983 Jun;3(6):1133-7. doi: 10.1128/mcb.3.6.1133-1137.1983.
9
Modulation of functional activities in cultured rat hepatocytes.培养大鼠肝细胞中功能活性的调节
Mol Cell Biochem. 1983;53-54(1-2):35-56. doi: 10.1007/BF00225245.
10
Plasma protein induction by isolated hepatocytes.分离的肝细胞诱导血浆蛋白生成
Mol Cell Biochem. 1983;53-54(1-2):89-109. doi: 10.1007/BF00225248.
J Biol Chem. 1951 Nov;193(1):265-75.
4
A modified technique of immunoelectrophoresis facilitating the identification of specific precipitin arcs.一种有助于识别特异性沉淀弧的改良免疫电泳技术。
J Immunol. 1960 Jan;84:93-7.
5
CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.哺乳动物细胞在化学成分明确的合成培养基中的克隆生长
Proc Natl Acad Sci U S A. 1965 Feb;53(2):288-93. doi: 10.1073/pnas.53.2.288.
6
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
7
SITE OF FORMATION OF THE GROUP-SPECIFIC COMPONENT AND CERTAIN OTHER SERUM PROTEINS.群体特异性成分及某些其他血清蛋白的形成部位。
Proc Soc Exp Biol Med. 1964 Apr;115:1005-7. doi: 10.3181/00379727-115-29101.
8
Clonal analysis of myogenesis.肌发生的克隆分析。
Science. 1963 Jun 21;140(3573):1273-84. doi: 10.1126/science.140.3573.1273.
9
The cultivation of human embryonic liver cells.人胚胎肝细胞的培养。
Exp Cell Res. 1962 Feb;26:9-36. doi: 10.1016/0014-4827(62)90199-4.
10
Production of serum albumin and of globulins by chick mesenchymal tissue and by HeLa tumour tissue in culture.鸡间充质组织和培养的海拉肿瘤组织产生血清白蛋白和球蛋白。
Nature. 1959 Aug 1;184(Suppl 6):361-2. doi: 10.1038/184361a0.