Awasthi L P
Arch Virol. 1981;70(3):215-23. doi: 10.1007/BF01315128.
An antiviral substance, showing highly significant virus inhibiting property, has been isolated from the aqueous extract of Cuscuta reflexa plants. The biologically active virus inhibitor was purified by fractionation with organic solvents followed by precipitation with a saturated solution of ammonium sulphate, dialysis, ultracentrifugation and filtration through a Sephadex G-200 column. It shows characteristics of proteins with a molecular weight around 14,000-18,000 daltons. The antiviral activity of the purified material was increased several folds. It has prevented the infection of several unrelated isometric as well as anisometric viruses in their hypersensitive and systemic hosts. Systemic resistance induced in lower treated as well as upper nontreated leaves of host plants, whose lower leaves had been treated with inhibitor, was significantly reversed in presence of Actinomycin D.
从菟丝子（Cuscuta reflexa）植物的水提取物中分离出了一种具有高度显著病毒抑制特性的抗病毒物质。通过用有机溶剂分级分离，随后用硫酸铵饱和溶液沉淀、透析、超速离心以及通过葡聚糖G - 200柱过滤，对这种具有生物活性的病毒抑制剂进行了纯化。它显示出分子量约为14,000 - 18,000道尔顿的蛋白质特征。纯化物质的抗病毒活性提高了数倍。它在过敏和系统宿主中阻止了几种不相关的等轴测以及非等轴测病毒的感染。在用抑制剂处理过下部叶片的宿主植物的下部处理叶片以及上部未处理叶片中诱导的系统抗性，在放线菌素D存在的情况下显著逆转。
