培养组织切片中软骨蛋白的代谢
Metabolism of cartilage proteins in cultured tissue sections.
作者信息
Paulsson M, Sommarin Y, Heinegård D
出版信息
Biochem J. 1983 Jun 15;212(3):659-67. doi: 10.1042/bj2120659.
Abstract
The biosynthesis and turnover of cartilage proteins was studied in organ cultures of bovine tracheal-cartilage sections. In cultures labelled with [3H]leucine, more than 99% of the labelled macromolecules were retained in the sections. About half of the [3H]leucine-labelled protein was extracted with 4M-guanidinium chloride. The incorporation of [3H]leucine into protein extractable with guanidinium chloride was linear with time, after an initial delay of 20-25 min. The 148kDa and 36kDa cartilage proteins were major labelled components in this extract. The elimination of the proteins was studied by using a pulse-chase protocol. The 148kDa protein was found to have a very slow turnover, similar to that of proteoglycans, whereas the 36kDa protein was eliminated more rapidly.
摘要
在牛气管软骨切片的器官培养中研究了软骨蛋白的生物合成和更新。在用[3H]亮氨酸标记的培养物中，超过99%的标记大分子保留在切片中。约一半的[3H]亮氨酸标记蛋白可用4M氯化胍提取。在用氯化胍可提取的蛋白中，[3H]亮氨酸的掺入在最初延迟20 - 25分钟后与时间呈线性关系。148kDa和36kDa的软骨蛋白是该提取物中的主要标记成分。通过脉冲追踪方案研究了这些蛋白的消除情况。发现148kDa蛋白的更新非常缓慢，类似于蛋白聚糖，而36kDa蛋白的消除更快。
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