6-磷酸葡萄糖酸脱氢酶对一种等排膦酸酯底物类似物的脱氢作用。
Dehydrogenation by gluconate 6-phosphate dehydrogenase of an isosteric phosphonate substrate analogue.
作者信息
Roach D J, Harrison R
出版信息
Biochem J. 1981 Sep 1;197(3):731-5. doi: 10.1042/bj1970731.
Abstract
6,7-Dideoxy-D-gluco-heptonic-7-phosphonic acid, the isosteric phosphonate analogue of gluconate 6-phosphate, was prepared by incubation of the corresponding analogue of glucose 6-phosphate with glucose 6-phosphate dehydrogenase and NADP+ in the presence of an enzyme NADPH-NADP+ recycling system. The analogue of gluconate 6-phosphate is a substrate for yeast gluconate 6-phosphate dehydrogenase, showing Michaelis-Menten kinetics at pH 7.5 and 8.0. At both pH values the Km values are approx. 3-fold higher and the Vmax. values approx. 7-fold lower than those of the natural substrate.
摘要
6,7-二脱氧-D-葡萄糖庚糖-7-膦酸，即6-磷酸葡萄糖酸的等排膦酸类似物，是通过在酶NADPH-NADP⁺循环系统存在的情况下，将6-磷酸葡萄糖的相应类似物与6-磷酸葡萄糖脱氢酶和NADP⁺一起孵育制备而成。6-磷酸葡萄糖酸的类似物是酵母6-磷酸葡萄糖脱氢酶的底物，在pH 7.5和8.0时呈现米氏动力学。在这两个pH值下，其Km值约比天然底物高3倍，Vmax值约比天然底物低7倍。
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