Carletti R, Spisani S, Traniello S
Boll Soc Ital Biol Sper. 1981 Nov 30;57(22):2257-63.
Casein, a phosphoprotein forming aggregates in solution, exerts chemotactic activity for human neutrophils. The protein was filtered on Sephadex G 100 to obtain fractions of homogeneous mol. weight and the column fractions were tested for chemotactic activity. The chemotactic activity was found only in the lighter peak, which was then digested by trypsin. The digested material was purified by filtration through AcA-54 and the 6.000 M.W. peptide containing the most phosphate groups and tyrosines resulted chemotactic for PMN. Most likely the phosphate groups are important in chemotactic recognition.
酪蛋白是一种在溶液中形成聚集体的磷蛋白，对人类中性粒细胞具有趋化活性。该蛋白在葡聚糖G 100上进行过滤以获得分子量均一的级分，并对柱级分进行趋化活性测试。趋化活性仅在较轻的峰中发现，然后用胰蛋白酶消化该峰。消化后的物质通过AcA - 54过滤进行纯化，含有最多磷酸基团和酪氨酸的6000分子量肽对多形核白细胞具有趋化作用。很可能磷酸基团在趋化识别中起重要作用。
