Meitner E R, Magdolenová S, Holan J
Z Mikrosk Anat Forsch. 1976;90(5):825-33.
The authors deal with the phenomenon of dualism of pale and dark ganglion cells in general, the phenomenon which, since FLEMMING (1882), still remains an actual problem of neurohistology. They deal with Purkinje cells from a special aspect with the aim to demonstrate the dualism through various staining methods. They directed their attention also to the question of the influence of perfusion and immersion fixation and length of staining with luxol-fast-blue on the production of luxol-positive (chromophilic) Purkinje cells. The authors have found that these methodologic circumstances do not influence the production of luxol-positive Purkinje cells, so it can be hardly spoken about an artifact. Examinations with the labelled leucine have shown that the increased metabolic activity can be observed in those Purkinje cells which in HE-picture are seen as pale ones. In the dark Purkinje cells leucine granulations are located on the surface of plasmatic membrane and they follow to some distance the main dendrite of those cells. The microautoradiographic method evidences for the increased leucine metabolism of the pale Purkinje cells as well.
作者总体上探讨了苍白和深色神经节细胞的二元性现象，自弗莱明（1882年）以来，这一现象仍是神经组织学的一个实际问题。他们从一个特殊的角度研究浦肯野细胞，旨在通过各种染色方法来证明这种二元性。他们还关注灌注和浸入固定以及用变色酸2R快速蓝染色的时长对产生变色酸2R阳性（嗜色性）浦肯野细胞的影响问题。作者发现这些方法学条件不会影响变色酸2R阳性浦肯野细胞的产生，因此很难说是一种假象。用标记亮氨酸进行的检查表明，在那些在苏木精-伊红染色图像中被视为苍白的浦肯野细胞中可以观察到代谢活性增加。在深色浦肯野细胞中，亮氨酸颗粒位于质膜表面，并在一定距离内沿着这些细胞的主要树突分布。显微放射自显影法也证明了苍白浦肯野细胞的亮氨酸代谢增加。
