Meitner E R
Z Mikrosk Anat Forsch. 1975;89(3):467-78.
The author refers about different staining of the Purkinje-cells with luxol-fast-blue, gallocyanin, thionin and toluidin blue, chrom-alum-hematoxylin-phloxin, impregnation according to Palmgren, lithium and iron-hematoxylin, combination of the staining with phloxin and the Palmgren-impregnation and about the different activity on the acid phosphatase. The phenomenon that in the same histological specimen the positive (dark, chromophile) and negative (light, chromophobe) cells are situated beside, is true for normal animals too, but the number of the dark Purkinje cells is conspicuous higher after stress situations (96-h. immobilisation, intermittent hypoxia). This finding interprets the author by the occurence of phospholipids by binding on the granulated endoplasmatic reticulum, but also as a property of the neuroplasm. The author emphasizes that the staining dualism "light -- dark" of the ganglion cells does not refer only to the ganglion cells of the spinal ganglions (et on some epithelial cells), but also on the Purkinje cells.
作者提到了用焦油紫、没食子蓝、硫堇和甲苯胺蓝、铬明矾苏木精-焰红染料对浦肯野细胞进行不同染色，按照帕尔姆格伦法进行浸染，锂和铁苏木精染色，焰红染料染色与帕尔姆格伦浸染相结合，以及它们在酸性磷酸酶上的不同活性。在同一组织学标本中，阳性（深色、嗜色性）细胞和阴性（浅色、嫌色性）细胞相邻存在的现象，在正常动物中也存在，但在应激情况（96小时固定、间歇性缺氧）后，深色浦肯野细胞的数量明显增多。作者将这一发现解释为磷脂通过结合在颗粒状内质网上的出现，也解释为神经浆的一种特性。作者强调，神经节细胞的“浅-深”染色二元性不仅适用于脊髓神经节的神经节细胞（以及一些上皮细胞），也适用于浦肯野细胞。
