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浦肯野细胞的不同染色性。

Different stainability of Purkinje cells.

作者信息

Meitner E R

出版信息

Acta Anat (Basel). 1977;97(2):191-9.

PMID:66846
Abstract

Regarding the different stainability when using the Luxol fast blue methods, two kinds of Purkinje cells of the rat are described: Luxol-positive and Luxol-negative cells. Since, by this method, phospholipids are demonstrated, the author suggests the prospective varying functional conditions of these cells. Different tinction of Purkinje cells has been confirmed also by other methods (gallocyanin-chromalaun, thionine, toluidine blue, lithium-haematoxylin, chromalaun-haematoxylin-phloxine and acid phosphatase) in both animal and human material. After 96 h of immobilization the different stainability of Purkinje cells becomes more marked, which penomenon can be as well explained with regard to the functional point of view. Similar differences, though less marked, were found also in neurosecretory cells of the nucelus supra-opticus of the rat and in the nuclear region of the ganglion semilunare Gasseri cells in man. Finally, the author refers to the relations between the Luxol blue staining method and Baker's method employing acid haematoxylin for demonstration of phospholipids in certain kinds of nervous system cells, taking into consideration Kroon's findings.

摘要

关于使用卢戈氏坚牢蓝方法时的不同染色性,描述了大鼠的两种浦肯野细胞:卢戈氏阳性细胞和卢戈氏阴性细胞。由于通过这种方法可显示磷脂,作者推测这些细胞可能存在不同的功能状态。在动物和人体材料中,其他方法(如间苯二酚蓝 - 铬明矾、硫堇、甲苯胺蓝、锂苏木精、铬明矾 - 苏木精 - 焰红染料和酸性磷酸酶)也证实了浦肯野细胞的不同染色情况。固定96小时后,浦肯野细胞的不同染色性变得更加明显,从功能角度来看,这一现象也可以得到很好的解释。在大鼠视上核的神经分泌细胞以及人类半月神经节加斯里细胞的核区域中也发现了类似的差异,不过不太明显。最后,作者参考克伦的研究结果,提及了卢戈氏蓝染色法与贝克氏用酸性苏木精显示某些神经系统细胞中磷脂的方法之间的关系。

相似文献

1
Different stainability of Purkinje cells.浦肯野细胞的不同染色性。
Acta Anat (Basel). 1977;97(2):191-9.
2
[Variable stainability of Purkinje cells].[浦肯野细胞的可变染色性]
Z Mikrosk Anat Forsch. 1975;89(3):467-78.
3
[Light and dark Purkinje cells (light microscopic and microautoradiographic studies].[明暗浦肯野细胞(光学显微镜和显微放射自显影研究)]
Z Mikrosk Anat Forsch. 1976;90(5):825-33.
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Pale and dark Purkinje cells (examination with Luxol fast blue and tritiated leucine).苍白和深色的浦肯野细胞(用卢氏固蓝和氚标记亮氨酸进行检测)
Folia Morphol (Praha). 1977;25(2):171-4.
5
[On the sex chromatin and sex chromatin-like nuclear structures in human Purkinje-cells].[关于人类浦肯野细胞中的性染色质及类性染色质核结构]
Beitr Pathol. 1975;154(3):243-55.
6
Histochemical and ultrastructural investigation of heterogeneous Purkinje neurons in mammalian cerebellum.哺乳动物小脑浦肯野神经元异质性的组织化学和超微结构研究。
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Detection of a new type of mouse eosinophil colony by Luxol-fast-blue staining.通过Luxol固蓝染色检测一种新型小鼠嗜酸性粒细胞集落。
Exp Hematol. 1980 May;8(5):549-61.
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Staining methods applied to glycol methacrylate embedded tissue sections.应用于甲基丙烯酸乙二醇酯包埋组织切片的染色方法。
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Determination of DNA surplus in the nuclei of some cerebellar Purkinje cells with different cytochemical methods.用不同细胞化学方法测定某些小脑浦肯野细胞核中的DNA剩余量。
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