Lewis S M, Skelly J V, Cousins S
Clin Lab Haematol. 1981;3(3):215-22. doi: 10.1111/j.1365-2257.1981.tb01335.x.
Platelet counting by means of the Coulter Thrombocounter, using the Thrombofuge method for specimen preparation has been evaluated in order to determine the cause of discrepancies in results obtained in interlaboratory quality assessment surveys. The method was assessed for accuracy, precision, drift and carry-over. When the method was used, as in routine practice, some discrepancies occurred; an important source of error was identified as being due to the level of sedimented plasma layer from which samples are removed. When the method was performed strictly in accordance with the manufacturer's instructions results were generally satisfactory and comparable to those obtained with the whole blood reference method, although, even with this standardized technique, the Thrombo-fuge tended to give higher platelet counts than the reference method with counts above 200 X 10(9)/l. This discrepancy to be independent of PCV, MCV or other identifiable factors.
为确定实验室间质量评估调查中所获结果存在差异的原因，对采用血栓离心机法制备标本、通过库尔特血小板计数器进行血小板计数的方法进行了评估。对该方法的准确性、精密度、漂移和残留进行了评估。在按照常规操作使用该方法时，出现了一些差异；已确定一个重要的误差来源是取样时沉淀血浆层的水平。当严格按照制造商的说明执行该方法时，结果总体令人满意，且与全血参考方法所获结果相当，不过，即使采用这种标准化技术，对于血小板计数高于200×10⁹/L的情况，血栓离心机法得出的血小板计数往往高于参考方法。这种差异与红细胞压积、平均红细胞体积或其他可识别因素无关。
