Schwartzman M, Pinkas R, Raz A
Eur J Pharmacol. 1981 Sep 11;74(2-3):167-73. doi: 10.1016/0014-2999(81)90527-6.
ATP and ADP stimulated the release of specific prostaglandin products from the perfused rabbit kidney heart. The two nucleotides produced the same qualitative profile of prostaglandin products. In kidney, prostaglandin E2 was the major product, whereas in heart 6-keto prostaglandin F1 alpha and prostaglandin E2 predominated. ATP was a slightly more potent than ADP. ATP administered into the perfused heart to kidney was rapidly hydrolyzed to ADP and AMP. The prostaglandin E2 generating activity of ATP was increased 6-10 fold when ATP was given together with AMP-PCP or AMP-PNP which competitively inhibit the activity of vascular ATPase. Thus, the rapid hydrolysis of ATP reduces its agonistic activity for prostaglandin release. ATP and ADP administered together at maximal stimulating doses produced an additive response for prostaglandin E2 release. These results and the results of tachyphylaxis experiments indicate that ATP and ADP interact independently with different types of purinergic receptors.
ATP和ADP刺激了灌注兔肾心脏中特定前列腺素产物的释放。这两种核苷酸产生的前列腺素产物具有相同的定性特征。在肾脏中，前列腺素E2是主要产物，而在心脏中，6-酮前列腺素F1α和前列腺素E2占主导地位。ATP的作用略强于ADP。注入灌注心脏-肾脏系统的ATP会迅速水解为ADP和AMP。当ATP与竞争性抑制血管ATP酶活性的AMP-PCP或AMP-PNP一起给药时，ATP生成前列腺素E2的活性增加了6至10倍。因此，ATP的快速水解降低了其对前列腺素释放的激动活性。以最大刺激剂量一起给药的ATP和ADP对前列腺素E2释放产生了相加反应。这些结果以及快速脱敏实验的结果表明，ATP和ADP与不同类型的嘌呤能受体独立相互作用。
