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20摄氏度储存对血浆中氨基酸浓度的影响。

Effect of storage at 20 degrees C on the concentration of amino acids in plasma.

作者信息

Ukida M, Schäfer K, Bode J C

出版信息

J Clin Chem Clin Biochem. 1981 Dec;19(12):1193-5. doi: 10.1515/cclm.1981.19.12.1193.

DOI:10.1515/cclm.1981.19.12.1193
PMID:7328386
Abstract

Amino acids were determined in whole and deproteinized plasma from six subjects, before and after storage at -20 degrees C over a period of four weeks. When the native plasma was stored the following changes were observed after four weeks (fractions, mean +/- SEM): increase of glutamic acid (to 1.30 +/- 0.11), decrease of glutamine (to 0.91 +/- 0.03), half-cystine (to 0.24 +/- 0.09) and arginine (to 0.94 +/- 0.02). In stored deproteinized plasma, only glutamine decreased slightly (to 0.96 +/- 0.02) in four weeks. The concentration of 22 other amino acids remained unchanged irrespective of the type of storage.

摘要

对六名受试者的全血和脱蛋白血浆中的氨基酸进行了测定,测定时间为在-20℃储存四周之前和之后。当天然血浆储存时,四周后观察到以下变化(组分,平均值±标准误):谷氨酸增加(至1.30±0.11),谷氨酰胺减少(至0.91±0.03),半胱氨酸减少(至0.24±0.09),精氨酸减少(至0.94±0.02)。在储存的脱蛋白血浆中,四周内只有谷氨酰胺略有下降(至0.96±0.02)。其他22种氨基酸的浓度无论储存类型如何均保持不变。

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Effect of storage at 20 degrees C on the concentration of amino acids in plasma.20摄氏度储存对血浆中氨基酸浓度的影响。
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