Heath D D, Parmeter S N, Osborn P J, Lawrence S B
J Parasitol. 1981 Dec;67(6):797-9.
A high level of resistance of oral infection with Echinococcus granulosus eggs was stimulated in lambs by two or more subcutaneous injections of oncospheres given 14 days apart. The degree of resistance was significantly higher than that resulting from a single injection. Resistance was apparently stimulated by the activated oncosphere or a stage of cyst development prior to 14 days of age. Studies on oncospheres cultured in vitro in sera collected from animals during immunization or after oral challenge showed that most cysts were killed before 7 days of culture had elapsed. This confirmed the observation that resistance was stimulated by an early stage of cyst development. The in vitro test also showed that two injections of oncospheres resulted in a marked increase in the lethal effects of serum. These lethal effects decreased with time, providing circumstantial evidence that the high degree of resistance stimulated by two or more injections may only be transient.
通过每隔14天皮下注射两次或更多次六钩蚴，在羔羊中激发了对细粒棘球绦虫卵口腔感染的高度抵抗力。这种抵抗力的程度明显高于单次注射所产生的抵抗力。抵抗力显然是由活化的六钩蚴或14日龄之前的囊肿发育阶段所激发的。对在免疫期间或口服攻击后从动物采集的血清中体外培养的六钩蚴的研究表明，大多数囊肿在培养7天之前就被杀死了。这证实了抵抗力是由囊肿发育的早期阶段所激发的这一观察结果。体外试验还表明，两次注射六钩蚴导致血清致死作用显著增加。这些致死作用随时间下降，间接证明两次或更多次注射所激发的高度抵抗力可能只是暂时的。
