Petersen N O, McConnaughey W B
J Supramol Struct Cell Biochem. 1981;17(3):213-21. doi: 10.1002/jsscb.380170303.
Fluorescence photobleaching recovery curves on a pair of membranes at various separations were calculated from a detailed knowledge of the variation in relative illumination areas and intensities as well as in relative contributions to the collected intensity with membrane separation. The observed diffusion coefficients were found to be relatively insensitive to membrane separation in all cases. Only for membranes with very different mobilities are there significant differences in fractional recoveries. Systematic variations in fractional recoveries with cell thickness may be indicative of differential mobility in the apical and basal membranes.
根据相对光照面积和强度的变化以及随着膜间距对收集强度的相对贡献的详细信息，计算了不同间距的一对膜上的荧光光漂白恢复曲线。在所有情况下，观察到的扩散系数对膜间距相对不敏感。只有对于迁移率非常不同的膜，分数回收率才有显著差异。分数回收率随细胞厚度的系统变化可能表明顶端膜和基底膜的迁移率不同。
